肥大细胞对THP-1巨噬细胞源性泡沫细胞胆固醇分配和流出的影响.ppt

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1、肥大细胞对THP-1巨噬细胞源性泡沫细胞胆固醇分配和流出的影响10/4/20211肥大细胞(MCs)一种参与炎症免疫应答反应的炎症细胞。胞浆内含有大量异染性颗粒（组胺等）。受刺激时脱颗粒,释放胞浆一系列可溶性介质到细胞外（compound48/80、oxLDL等可以刺激MCs脱颗粒)。研究背景10/4/20212促动脉粥样硬化(As)形成MCs加速oxLDL诱导平滑肌细胞泡沫化。Compound48/80处理的新西兰白兔和apoE-/-鼠主动脉脂纹形成比对照组早，面积大，分布广。10/4/202

2、13HDLMetabolismandReverseCholesterolTransport胆固醇流出减少A-ICECECEFCFCLCATFCBileSR-BIA-IABCA1MatureHDLNascentHDLMC?ABC1=ATP-bindingcassetteprotein1;A-I=apolipoproteinA-I;CE=cholesterylester;FC=freecholesterol;LCAT=lecithin:cholesterolacyltransferase;

3、SR-BI=scavengerreceptorclassBI10/4/20214实验结果ABTheeffectofcompound48/80onMCsdegranulationMCswerestainedbytoluidineblue(×1000)A，MCs；B，MCs+C48/80.例数组胺含量（µg/ml）组胺释放率（n）颗粒释放液细胞裂解液（%）50.340±0.0050.077±0.02181.53±4.51MCshistaminereleasepercentage10/4/20215Fo

4、rmationofTHP-1macrophage-derivedfoamcellsCE/TCRatio:68.7%HPLCanalysisofTHP-1macrophage-derivedfoamcellsOilredOstainingofTHP-1macrophage-derivedfoamcellsFC，freecholesterolCE，cholesterolesterFCCE10/4/20216TheeffectofMCsontheaccumulationofcellcholesterol

5、inTHP-1macrophage-derivedfoamcellsGroupsTCFCCEcontrol1092.3±65336.3±31756±60HDL3527.3±49a276.9±34250.4±17MCs891.3±62650.1±46b241.2±19bHDL3+MCs917.9±14c848.5±16c69.4±2cC48/80807.3±68343.1±36464.2±13HDL3+C48/80314.3±10a253.2±1161.1±8Valuesarerepresented

6、as（）mgcholesterol/gprotein,(n=5).a:P<0.05vs.control,HDL3+MCsgroup;b:P<0.05vs.controlgroup;c:P<0.05vs.HDL3group.10/4/20217CholesterolstandardcontrolHDL3MCsHDL3+MCsC48/80HDL3+C48/80ThechromatographypictureofcholesterolandcholesterolesterinTHP-1macrophag

7、e-derivedfoamcellsidentifiedbyhighperformanceliquidchromatography.10/4/20218TheeffectofMCsonthedistributionofcellfreeandesterifiedcholesterolinTHP-1macrophage-derivedfoamcellscontrol(alone)=69.2%;withHDL3=47.5%;withMCs=27.1%;withHDL3+MCs=7.6%;withcomp

8、ound48/80=57.5%;andwithcompound48/80+HDL3=19.4%.a:P<0.05vs.controlgroup;b:P<0.001vs.HDL3group.n=5.10/4/20219TheeffectofMCsoncholesteroleffluxfromTHP-1macrophage-derivedfoamcellsmediatedbyHDL3control=2.89%;withHDL3=40.98%;withMCs=2.91%;withHDL3