肥大细胞对THP-1巨噬细胞源性泡沫细胞胆固醇分配和流出的影响PPT课件.ppt

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1、肥大细胞对THP-1巨噬细胞源性泡沫细胞胆固醇分配和流出的影响赵战芝杨永宗南华大学心血管病研究研6/11/20211肥大细胞(MCs)一种参与炎症免疫应答反应的炎症细胞。胞浆内含有大量异染性颗粒（组胺等）。受刺激时脱颗粒,释放胞浆一系列可溶性介质到细胞外（compound48/80、oxLDL等可以刺激MCs脱颗粒)。研究背景6/11/20212促动脉粥样硬化(As)形成MCs加速oxLDL诱导平滑肌细胞泡沫化。Compound48/80处理的新西兰白兔和apoE-/-鼠主动脉脂纹形成比对照组早，面积大，分布广。6/11/20

2、213HDLMetabolismandReverseCholesterolTransport胆固醇流出减少A-ICECECEFCFCLCATFCBileSR-BIA-IABCA1MatureHDLNascentHDLMC?ABC1=ATP-bindingcassetteprotein1;A-I=apolipoproteinA-I;CE=cholesterylester;FC=freecholesterol;LCAT=lecithin:cholesterolacyltransferase;SR-BI=scavenger

3、receptorclassBI6/11/20214实验结果ABTheeffectofcompound48/80onMCsdegranulationMCswerestainedbytoluidineblue(×1000)A，MCs；B，MCs+C48/80.例数组胺含量（µg/ml）组胺释放率（n）颗粒释放液细胞裂解液（%）50.340±0.0050.077±0.02181.53±4.51MCshistaminereleasepercentage6/11/20215FormationofTHP-1macrophage-derive

4、dfoamcellsCE/TCRatio:68.7%HPLCanalysisofTHP-1macrophage-derivedfoamcellsOilredOstainingofTHP-1macrophage-derivedfoamcellsFC，freecholesterolCE，cholesterolesterFCCE6/11/20216TheeffectofMCsontheaccumulationofcellcholesterolinTHP-1macrophage-derivedfoamcellsGroupsTCFCCEc

5、ontrol1092.3±65336.3±31756±60HDL3527.3±49a276.9±34250.4±17MCs891.3±62650.1±46b241.2±19bHDL3+MCs917.9±14c848.5±16c69.4±2cC48/80807.3±68343.1±36464.2±13HDL3+C48/80314.3±10a253.2±1161.1±8Valuesarerepresentedas（）mgcholesterol/gprotein,(n=5).a:P<0.05vs.control,HDL3+MCsgro

6、up;b:P<0.05vs.controlgroup;c:P<0.05vs.HDL3group.6/11/20217CholesterolstandardcontrolHDL3MCsHDL3+MCsC48/80HDL3+C48/80ThechromatographypictureofcholesterolandcholesterolesterinTHP-1macrophage-derivedfoamcellsidentifiedbyhighperformanceliquidchromatography.6/11/20218The

7、effectofMCsonthedistributionofcellfreeandesterifiedcholesterolinTHP-1macrophage-derivedfoamcellscontrol(alone)=69.2%;withHDL3=47.5%;withMCs=27.1%;withHDL3+MCs=7.6%;withcompound48/80=57.5%;andwithcompound48/80+HDL3=19.4%.a:P<0.05vs.controlgroup;b:P<0.001vs.HDL3group.n

8、=5.6/11/20219TheeffectofMCsoncholesteroleffluxfromTHP-1macrophage-derivedfoamcellsmediatedbyHDL3control=2.89%;withHDL3=40.98%;withM