胶质瘤光动力疫苗的体外细胞毒性实验研究

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1、·80·中国微侵袭神经外科杂志2015年02月20日第20卷第2期ChinJMinimInvasiveNeurosurg,VOL.20,NO.2,February20,2015·实验研究·胶质瘤光动力疫苗的体外细胞毒性实验研究袁开长，杨非，赵飞，林彬，李方成【摘要】目的制备胶质瘤光动力疫苗并检测体外抗肿瘤效应。方法采用4种不同方法制备胶质瘤疫苗。冻融疫苗：冻融法制备人胶质瘤细胞U251抗原，呈递给树突状细胞（DC）制备疫苗。热休克疫苗：热休克法处理人胶质瘤细胞U251并提取抗原，呈递给DC制备疫苗。光动力全细

2、胞抗原疫苗：光动力法提取人胶质瘤细胞U251全细胞抗原并呈递给DC制备疫苗。光动力洗脱抗原疫苗：光动力法处理人胶质瘤细胞U251后，再用弱酸洗脱法提取活细胞表面抗原，呈递给DC制备疫苗。将上述4种不同方法制备的疫苗在体外与自体淋巴细胞以及靶细胞U251反应，然后用MTT法检测靶细胞凋亡率及坏死率，比较4种疫苗的体外抗肿瘤细胞效应。结果冻融疫苗、热休克疫苗、光动力全细胞抗原疫苗和光动力洗脱抗原疫苗的靶细胞死亡率分别是：（10.02±0.32）%、（18.34±0.46）%、（35.56±1.75）%和（54.4

3、5±2.14）%，4组疫苗抗肿瘤细胞效应是光动力洗脱抗原疫苗＞光动力全细胞抗原疫苗＞热休克疫苗＞冻融疫苗。结论胶质瘤光动力洗脱抗原疫苗是一种具有良好体外抗肿瘤效应的新型疫苗。【关键词】神经胶质瘤；化学疗法；疫苗；光动力；细胞毒性中图分类号：R730.264文献标志码：Adoi：10.11850/j.issn.1009-122X.2015.02.012Invitrocytotoxicityofgliomaphotodynamicvaccine:anexperimentalstudyYuanKaizhang1,Y

4、angFei1,ZhaoFei1,LinBin1,LiFangcheng21.DepartmentofNeurosurgery,theThirdAffiliatedHospitalofAnhuiMedicalUniversity,HefeiBinhuHospital,Hefei,Anhui230601,China;2.DepartmentofNeurosurgery,theSecondAffiliatedHospitalofSunYat-SenUniversity,Guangzhou,Guangdong510

5、120,ChinaAbstract:ObjectiveTopreparegliomaphotodynamicvaccinesanddetecttheiranti-tumoreffectsinvitro.MethodsFourkindsofgliomaspecifictumorvaccineswerepreparedbydifferentmethods.Freeze-thawcellvaccine(No.1):whole-cellantigensofhumangliomacellslineU251wereext

6、ractedusingfreeze-thawmethodtoproducedendriticcell(DC)vaccine.Heatshocktumorvaccine(No.2):antigensofhumangliomacelllineU251wereextractedafterheatshockprocessing,whichwerepresentedtoDCforvaccines.Photodynamicwhole-cellantigentumorvaccine(No.3):humangliomacel

7、llineU251cellsasantigenwerepresentedtoDCtoproducevaccinesbyphotodynamicextractionmethod.Photodynamicelutionantigentumorvaccine(No.4):U251cellswerecultivatedwithphotosensitizersand,aftertreatmentbyphotodynamicmethod,elutedbyweakacidsolutiontoextractantigensf

8、orpreparingDCvaccine.Tocomparetheeffectsofanti-cancerinvitro,the4vaccinesweremixedandreactedrespectivelywithhostlymphocytesandU251cells,andtheapoptosisandnecrosisratesoftheU251cellsweredetectedbyMTTass