Synthesis of Functionalized ZnSe Nanoparticles and Their Applications in the Determination of Bovine Serum AIbumin

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1、JFluoresc(2009)19:437–441DOI10.1007/s10895-008-0430-2ORIGINALPAPERSynthesisofFunctionalizedZnSeNanoparticlesandTheirApplicationsintheDeterminationofBovineSerumAlbuminLiLi&FenfenZhang&YapingDing&YapingWang&LuluZhangReceived:1June2008/Accepted:14October2008

2、/Publishedonline:30October2008#SpringerScience+BusinessMedia,LLC2008AbstractLuminescentquantumdots(QDs)-semiconductorandcontrollableandnarrowemissionbandscomparedwithnanocrystalswerepromisingalternativetoorganicdyesforthetraditionalorganicfluorescentdyes[

3、13],sotheyarefluorescence-basedapplications.Inthispaper,wedevel-idealfluorescentdyestoreplaceorganicfluorescentdyes.opedprocedurestousemercaptoaceticacid(MAA)toSincethereportontheaqueoussynthesisofmercaptoetha-modifyZnSenanoparticlesandmadethenanoparticle

4、stonol—andthioglycerol—cappednanocrystalswasappearedbesolubleforthequantitativeandselectivedeterminationin1996[14],solubilityofQDshasbeenresolved,anditofbovineserumalbumin(BSA).Maximumfluorescencehasbeenwidelyusedinbioanalytics.intensitywasproducedatpH7.0

5、,withexcitationandSerumalbuministhemostabundantproteinintheemissionwavelengthsat242and348nm,respectively.circulatorysystem,andithasmanyphysiologicaland△Underoptimalconditions,thestraightlineequation:F=pharmacologicalfunctions.Itisabletocarrydrugsaswell0.3

6、8+0.34C(μg/ml)wasfoundbetweentherelativeasendogenousandexogenoussubstances.AndnumerousfluorescenceintensityandtheconcentrationofBSAintheexperimentswiththeaimofcharacterizingthebindingrangeof9.6–124.8μg/ml,andthelimitofdetectionwascapacityandsitesofalbumin

7、shavebeencarriedout[15,2μg/ml.16].ThemostimportantpropertyofserumalbuministhatitservesasadepotproteinandasatransportproteinforaKeywordsQuantumdots.Spectrofluorimetricdetection.varietyofcompound.Bovineserumalbumin(BSA)hasZnSenanoparticles.Bovineserumalbumi

8、nbeenoneofthemostextensivelystudiedofthisgroupofproteins.TherehavebeenmanymethodsreportedtodetermineIntroductionbovineserumalbumin,suchasresonancelightscatteringtechniques[17–19],chemiluminescence[20],BradfordSemico