中国主要水果抑制肝癌HepG2细胞和结肠腺癌Caco-2细胞增殖活性评价

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1、现代食品科技ModernFoodScienceandTechnology2015,Vol.31,No.4中国主要水果抑制肝癌HepG2细胞和结肠腺癌Caco-2细胞增殖活性评价刘冬，万红霞，孙海燕（深圳职业技术学院深圳市发酵精制检测系统重点实验室，广东深圳518055）摘要：分别采用Folin-Ciocalteu法测定了水果提取物的总酚含量，采用亚甲基蓝法测定了其抗人肝癌细胞HepG2和人结肠腺癌细胞Caco-2增殖的活性，分析了总酚含量与抗HepG2和Caco-2细胞增殖活性之间的相关性。结果显示，25种水果中李子的总酚含量最高（1686.08±96.94molGAE/100

2、鲜果），西瓜（83.54±2.10molGAE/100g鲜果）和哈密瓜（79.35±0.76molGAE/100g鲜果）的总酚含量最低；在可测出抗增殖EC50值的水果中，李子（18.99±0.06mg/mL）和番石榴（20.94±0.09mg/mL）抗HepG2细胞增殖的活性最强，梨的活性最弱（389.63±10.82mg/mL）。李子抗Caco-2细胞增殖的活性最强（8.73±0.11mg/mL），火龙果的活性最弱22（388.07±7.04mg/mL）。水果的抗HepG2和Caco-2细胞增殖活性与其总酚含量相关性显著（R=0.4147，p<0.01；R=0.4071，p

3、<0.05），该相关性表明水果中的多酚具有良好的抗肿瘤细胞增值的活性。关键词：水果；多酚；人结肠腺癌Caco-2细胞；人肝癌HepG2细胞；抗增殖活性文章篇号：1673-9078(2015)4-23-28DOI:10.13982/j.mfst.1673-9078.2015.4.005EvaluationoftheAnti-proliferativeActivitiesofMajorFruitsinChinaonHumanHepaticCarcinoma(HepG2)andColonAdenocarcinoma(Caco-2)CellsLIUDong,WANHong-xia,SUN

4、Hai-yan(ShenzhenKeyLaboratoryofFermentation,PurificationandAnalysis,Shenzhenpolytechnic,Shenzhen518055,China)Abstract:Thetotalphenoliccontentandtheanti-proliferativeactivitiesofthephenolicextractsagainstHepG2andCaco-2cellsweredeterminedbytheFolin-Ciocalteumethodandthemethyleneblueassay,respec

5、tively;thecorrelationbetweenthetotalphenoliccontentandtheanti-proliferativeactivityoftheextractswasanalyzed.Theresultsoftheseanalysesrevealedthatplum(PrunussalicinaLindl.)hadthehighesttotalphenoliccontent(1686.08±96.94molgallicacidequivalent(GAE)/100goffreshfruit),whereaswatermelon(Citrullus

6、lanatus)(83.54±2.10molGAE/100goffreshfruit)andhamimelon(Cucumismelovar.saccharinus)(79.35±0.76molGAE/100goffreshfruit)hadthelowestphenoliccontentamongthe25testedspecies.Amongthefruitspecieswithquantifiableanti-proliferativeactivities(halfmaximaleffectiveconcentration(EC50)),plumandguava(Psid

7、iumguajavaLinn.)showedthestrongestanti-proliferativeactivitiesagainstHepG2cells(EC50values:18.99±0.06and20.94±0.09mg/mL,respectively),whereaspears(Pyrusspp.)showedtheweakestanti-proliferativeactivityagainstthesecells(EC50value:389.63±10.82mg/