归肝经中药对肝癌hepg2细胞增殖抑制与凋亡诱导作用.研究

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1、ExperimentalstudyonthedominanceofproliferationinhibitionandapoptosisinducingofChineseherbswithliverchannel-tropisminHepG2ABSTRACTTutor:XiaoZhuMaster:HuangLizhongProfessorandTianXuefeiProfessor(HunanUniversityofTraditionalChineseMedicine,410208)Thehepatocarci

2、nomaoccupiesthesecondrateofcancerinChina.Atpresent,therewerenospecialdrugstotreatlate,midtrimesterandrecurrenthepatocarcinoma.TheChinesemedicinewasreceivedgreatattentionforimprovingclinicalsymptomsandlifequality,prolongingsurvivaltimesinclinicalanti-hepatoca

3、rcinomatherapy.InrecentyearstheChinesemedicinehadmadesomeprogressesinclinicalanti-hepatocarcinoma.However,itsmechanismofanti-hepatocarcinomawasstillnotcompletelydefined.Accordingly,researchingontheanti-hepatocarcinomamechanismofChinesemedicineandseekingtheef

4、fectivecomponentshadimportantsignificanceintheexplorationofnewtherapeuticmethods.Purpose:ToobservetheeffectofthetraditionalChinesemedicineascribingtothelivermeridianincellmultiplicationandapoptosisofHepG2in-vitroandinitiallytodiscussthetrendoftheanti-hepatoc

5、arcinomaeffecttotheChinesemedicineascribingtothelivermeridian.Methods:1.ZedorayRhizome,LeechandSalviaascribingtothelivermeridianwereselectedtocomparewithHedyoticdiffusaascribingtothenon-livermeridian.ZedoaryTurmericOilwasabstractedbyelaeo-volatilizedway,Salv

6、iaandHedyoticdiffusawaterextractwereabstractedbythemethodofaquafer-extrac＆alcohol-sinked.Leechwaterextractwasabstractedbythemethodofaquafer-extracted＆alcohol-sinkedandtacho-freeze-thawinginliquidnitrogen.SalviaandHedyoticdiffusawaterextractcontentwereidentif

7、iedbyHighperformanceliquidchromatography（HPLC）.ZedorayRhizomeOilwasassayedbyelaeo-volatilizedway.ThetwopreparationmethodsofLeechwaterextractwerecontrasted.2.UsingMTTassayedmultiplicationandinhibitionofZedorayRhizome,LeechandSalviaextractascribingtotheliverme

8、ridianonHepG2cellswhichcomparedwithHedyoticdiffusaextractascribingtothenon-livermeridianandblankcontrolgroup.Accordingtolinearregressionequationcalculatedthedrugconcentrationandcontentoftheextra