归肝经中药对肝癌HepG2细胞增殖抑制与凋亡诱导作用分析

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1、-------ExperimentalstudyonthedominanceofproliferationinhibitionandapoptosisinducingofChineseherbswithliverchannel-tropisminHepG2ABSTRACTTutor:XiaoZhuMaster:HuangLizhongProfessorandTianXuefeiProfessor(HunanUniversityofTraditionalChineseMedicine,410208)Thehepatocarcinomaoccupiesthesecondrateofcan

2、cerinChina.Atpresent,therewerenospecialdrugstotreatlate,midtrimesterandrecurrenthepatocarcinoma.TheChinesemedicinewasreceivedgreatattentionforimprovingclinicalsymptomsandlifequality,prolongingsurvivaltimesinclinicalanti-hepatocarcinomatherapy.InrecentyearstheChinesemedicinehadmadesomeprogresses

3、inclinicalanti-hepatocarcinoma.However,itsmechanismofanti-hepatocarcinomawasstillnotcompletelydefined.Accordingly,researchingontheanti-hepatocarcinomamechanismofChinesemedicineandseekingtheeffectivecomponentshadimportantsignificanceintheexplorationofnewtherapeuticmethods.Purpose:Toobservetheeff

4、ectofthetraditionalChinesemedicineascribingto-----------thelivermeridianincellmultiplicationandapoptosisofHepG2in-vitroandinitiallytodiscussthetrendoftheanti-hepatocarcinomaeffecttotheChinesemedicineascribingtothelivermeridian.Methods:1.ZedorayRhizome,LeechandSalviaascribingtothelivermeridianwe

5、reselectedtocomparewithHedyoticdiffusaascribingtothenon-livermeridian.ZedoaryTurmericOilwasabstractedbyelaeo-volatilizedway,SalviaandHedyoticdiffusawaterextractwereabstractedbythemethodofaquafer-extrac＆alcohol-sinked.Leechwaterextractwasabstractedbythemethodofaquafer-extracted＆alcohol-sinkedand

6、tacho-freeze-thawinginliquidnitrogen.SalviaandHedyoticdiffusawaterextractcontentwereidentifiedbyHighperformanceliquidchromatography（HPLC）.ZedorayRhizomeOilwasassayedbyelaeo-volatilizedway.ThetwopreparationmethodsofLeechwaterextractwerecontrasted.2.UsingMTTassayedmultiplicationandinhibitionofZed

7、orayRhizome,LeechandSalviaextractascribingtothelivermeridianonHepG2cellswhichcomparedwithHedyoticdiffusaextractascribingtothenon-livermeridianandblankcontrolgroup.Accordingtolinearregressionequationcalculatedthedrugconcentrationan