海藻糖和透明质酸对生物分子和微生物的保护及其作用机制

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1、中国农业大学博士学位论文海藻糖和透明质酸对生物分子和微生物的保护及其作用机制姓名：张玉华申请学位级别：博士专业：食品科学指导教师：籍保平;凌沛学20070601AbstractTrehaloseisanon-reducingdisaccharidewithparticularbiologicalactivityofpreservingbiomoleculesduringfreezinganddehydration．Researchisfocused011itsrolerecently．Hyaluronicacido{A)isakindofpolysacch

2、aridewithlinearchainexistinginintercellulm"substanceofanimals．Theprotectiveeffectsandmechanismsoftrehalose．HAandtheirco血bin撕onOnbiomoloculesandmicrobeswefeinvestigated．TheinvestigationwilloffertheoreticalbaseforapplicationoftiehaloseandHAasprotcctants．TheplDlB嘶veeffectsandmechanis

3、msoftrebalose,HAandtheircombination011thestabilizationofamodelprotein,pancreatickininogenase0'Kase)．werea∞Inine正Duringrefrigeration,repeatedfreeze-thawing，freeze-drying。vaconm-dryingand7weeksofstorageat45℃，thestabilizationoftrehalose011PKageactivitywagsignmcant,andHAaloneWagless-e

4、ffecfive．However,astrongeriR'otectinnofactivityinfreeze-driedPKmeWagobtainedwhentrehaloseandHAusedtogetherthaneachagentused910llS,Theaw,ondderivativeinfraredspectraofPKasein曲曙am／deIregion啊啪measured．ItWagfoundthattherewereseveralalterationsinthesecondaryglructur@ofPKageduringfreeze

5、-dryingandstorage砒45℃relativetothatofthenative．aqueottsprotm．Trehaloseandthecombinationofu'chaloscandHApreservedtheInmnative-likesecondarysm】c-mreofPKasethanthatofPKasedriedaloneorinpl∞SellCeOfHA．Thereishna_c‰bftween-OHgroupsoftrehaloseandthepolHgroupsofPKaseafterlyophilization,wh

6、ichisformedviahydrogenbonding．TrehalosetakesplaceofthemissmgboundwatersurroundingthepolargroupsofPKase．Therefore，theⅫ,ndrystructmeofPKaseisstabilized．ThereisnointeractionbetweenHAandPKase，therefore，HAislessactiveinpreservingnativeprotciJLTrehaloseandHAoffercomplementary讲qⅪ币∞whenth

7、etwotypesofadditivesaleusedtogether．TrehalosepreservesPKasenativesu'ucturebyhydrogenbonditlg．AdditionofHAincreasestheglass-transitiontemperature(坳ofPKaseformulationinpresenceoftrehalose．TheprotectiveeffectsandmechanismsoflrehaloseandHAandtheircombinationonthestabilizationofamodelm

8、embranelipidbilayer,liposomes，Wel