慢病毒介导的rna干扰rrs1基因对肝癌smmc-7721细胞生物学行为的影响

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1、分类号：密级：学号：20121270单位代码：10092HEBEINORTHUNIVERSITY硕士学位论文慢病毒介导的RNA干扰RRS1基因对肝癌SMMC-7721细胞生物学行为的影响单位：河北北方学院专业：外科学研究生姓名：王继涛导帅姓名及职称:孙百军教授研究起止日期：2013.2-2015.2提交日期：2015.5分类号：密级：单位代码：10092学号：20121270慢病毒介导的RNA干扰RRS1基因对肝癌SMMC-7721细胞生物学行为的影响单位：河北北方学院专业：外科学研究生姓名：王继涛导师姓名及职称：孙百军

2、教授研究起止日期：2013.2-2015.2提交日期：2015.5目录中文摘要····································································································1英文摘要····································································································3英文缩写··············

3、······················································································6研究论文慢病毒介导的RNA干扰RRS1基因对肝癌SMMC-7721细胞生物学行为的影响前言··································································································7材料与方法···························

4、·····························································8结果································································································21附图································································································23附

5、表································································································26讨论································································································27结论······························································

6、··································33参考文献··························································································34综述核糖体蛋白及其调控蛋白与肿瘤关系的研究进展················40致谢························································································

7、················50个人简历··································································································51I中文摘要慢病毒介导的RNA干扰RRS1基因对肝癌SMMC-7721细胞生物学行为的影响摘要肝癌是世界最常见的恶性肿瘤之一，在世界恶性肿瘤中发病率第五，死亡率第三，其发病率和死亡率逐年增加。肝癌早期临床症状不典型，但病程发展迅速，死亡率极高，使人类的健康受到了极大的挑战。RNA干扰

8、（RNAinterference,RNAi）是由双链RNA介导的基因沉默现象,通过基因工程技术将外源或内源的短发夹RNA（shorthairpinRNA，shRNA）导入目的细胞,能够靶向降解目的基因mRNA，可阻断靶基因的功能表达,进而导致转录后基因沉默。RNA干扰技术的不断进步能够为恶性肿瘤的早期诊断及靶向治疗提