yc-1增强乏氧人肺腺癌a549细胞放射敏感性的作用机制分析

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1、徐州医学院硕士学位论文次之，m．Y。l玎下降最少，但其表达仍远低于H+RT组。结论YC．1增强乏氧人A硼细胞放射敏感性的机理与其能够抑制HIF-la及其下游靶基因VEGF的表达密切相关；不同时序YC．1联合放疗对HIF．1a的表达的影响不相同，初步可确立首先放疗的模式。关键词3．(5。．羟甲基2t-呋喃基)．1．苯甲基吲唑；乏氧；As9细胞系；乏氧诱导因子．1a；放射增敏4徐州医学院硕士学位论文ThemechanismofYC-1enhanceseffectofradiationinhypoxlchumanpulmonarVadenocarclnomaA549ceHs一‘一●‘’’Ab

2、stractObjectiveToexplortherelationshipofradiosensitiveeffectsofYC-1onhopxicadenocarcinomaA549celllineandHIF—laanditstargetgeneVEGF．Methods①ⅣrrTassayWasusedtotesttheinhlbitoryeffectofCoCkonA549celllineproliferation-@)A549cellsweredividedinto3groups．O)treatedwithdiverseconcentratiOIlSofCoCk：0，75，1

3、50，300，450I_tmol／L；(2)treatedwithdifferentconcentrationsofYC-linhypoxia．9，l，10，50，100btmol／L；(3)treatedwithirradiation：radiationaloneinnormoxia(RT)，radiationinhypoxia(H+RT)，radiationcombinedwithYC一1：YC一1Wastreatedin24hattertheradiationinhypoxia(H．帐nY)，YC-1wasfirsttreatedinhypoxia24handthenaccept

4、edtheradiation(H．Ⅳ?RT)，YC-1was仃eatedin30rainbeforeacceptedtheradiation口+Y+RT)．Semi-quantitativeRT-PCR,WesternBlottingwereusedtodetccttheH匝一laanditstargetgenes’mRNAandproteinlevelsindifferentgroupsafterculturedfor钝，24hand48h．③ImmunofluorescencestainingwasusedtodetecttheexpressionofHIF-laaftercult

5、uredfor24h．ResultsCoCkcouldinhibitthegrowthofA549cellsinatime-dose-dependentnlanner．RT-PCRandWesternBlotshowedthatA549cellshadsomeexpressionsofHIF-la,VEGFinnormoxia,andCoCkcouldenhancedtheirexpressionsinthetime-dosedependence．YC-1couldn'tinhibittheHIF—lamRNAexpressionindifferentconcentrationsofY

6、C-l舶m4hto48h．However,YC—lcouldinhibittheexpressionsofHIF—laprotein,VEGFmRNAandproteinintime-dosedependence．XraycouldiuducethehighexpressionofHIF-laanditstargetgeneVEGFintimedependence．InthegroupofH+Y-啄T，theexpressionofHIF-1Waslowest，followedbyH+RT?Ygroup，andthehighestWasH+Y?RT．ConclusionThemecha

7、nismofYC—lcouldenhancehypoxicA549cellradiosensitivityiscloselycon'elatedwiththatYC—lcouldinhibittheexpressionsofHⅡ-laanditstargetgeneVEGF．KeywordsYC—l；Hypoxia；．A蟮49cellline；Hypoxia-induciblefactor-1；Hypoxiaradiosensitivity；5