mir-18a对肺腺癌a549细胞放射增敏作用与分子机制分析

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1、第三军医大学硕士学位论文英文缩写词一览表ATMataxia-telangiectasiamutated共济失调-毛细血管扩张症突变基因ATRATMandRad3-relatedgeneATM及Rad3相关基因BCRPbreastcancerresistanceprotein乳腺癌耐药蛋白bpbasepair碱基对cDNAcomplementaryDNA互补DNACSCcancerstemcell肿瘤干细胞DDRDNA-damageresponseDNA损伤反应DMSOdimethylsulfoxide二甲基亚砜dNTPdeoxyribonucle

2、oside脱氧核糖核苷三磷酸DOXdoxorubincin多柔比星DSBsdoublestrandbreaksDNA双链断裂EDTAethylenediaminetetraaceticacid乙二胺四乙酸GAPDHglyceraldehyde-3-phosphatedehydrogenase甘油醛-3-磷酸脱氢酶HRPhorseradishperoxidase辣根过氧化物酶kDkilodalton千道尔顿mRNAmessengerribonucleicacid信使核糖核酸mTORmammaliantargetofrapamycin哺乳动物雷帕霉素

3、靶蛋白NSCLCnon-smallcelllungcancer非小细胞肺癌ODopticaldensity光密度OPTI-MEMOPTI-ModifiedEagleMedium改良型MEM培养基PAGEpolyacrylamidegelelectrophoresis聚丙烯酰胺凝胶电泳PBSphosphatebufferedsaline磷酸盐缓冲液PCRtranscription-polymerasechainrection聚合酶链式反应PMSFphenylmethanesulfonylfluoride苯甲基磺酰氟 PVDFpolyvinylide

4、nedifluoride聚偏（二）氟乙烯Real-timereal-timepolymerasechainreaction实时聚合酶链反应PCR1第三军医大学硕士学位论文rpmrotationperminute转/分钟RPMI1640RoswellParkMemorialInstitute1640RPMI1640细胞培养液RT-PCRreversetranscription-polymerasechainreaction反转录-多聚酶链式反应SDSsodiumdodecylsulfate十二烷基磺酸钠TBStrisbufferedsalineTr

5、is缓冲盐TBSTtrisbufferedsaline/withTween20Tris磷酸盐溶液/吐温20TEMEDtetramethylethylenediamine四甲基乙二胺TICstumor-initiatingcells肿瘤起始细胞Tristris(hydroxylmethyl)aminomethane三羟甲基氨基甲烷2第三军医大学硕士学位论文ResearchonmiR-18aenhancingradiosensitivityofA549cellsAbstractObjective:Lungcanceristhemostcommonca

6、ncerintheworld.Approximately1.6millioncasesoflungcancerhaveoccurredin2008,ofwhich80%werenon-smallcelllungcancer(NSCLC)patients.SurgicalresectionisknownasthemosteffectivetreatmentforNSCLC,butduetothefactthatmostdiagnoseswereconfirmedinanadvancedstage,onlyafewpatientscanbecured

7、bysurgicaltreatment,andmorethan70%patientsneedradiotherapyinthetreatment.However,recurrenceisobservedinpatientsundergoingradiotherapy,whicheventuallyleadstodeath.Studiesshowthatradiationresistanceisthekeytothefailureofradiotherapy.Therefore,exploringthemechanismunderlyingradi

8、ationresistanceisnotonlyimportantformoreeffectiveradiotherapy,butals