glu-gnps对人肺腺癌细胞株a549放射增敏作用的初步分析

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1、LJ东人学硕十学位论文作用后，细胞周期的分布发生变化，S期细胞所占比例由24．11+6．1％降低到13．68+0．22％(P<0．05)，G2／M期细胞所占比例则由6．53+2．05％上升到13．01+1．15％(P<0．05)。Glu．GNPs、单纯放射均可诱导A549细胞凋亡，凋亡率分别为7．64+1．43％、13．46+1．99％(P<0．01VS对照组)，联合放射组凋亡率为21．43士1．04％，显著高于前两组(P

2、生长抑制率逐渐增加。纳米金处理后，可促使A549细胞由放射抗拒的S期向放射敏感的G2／M期转换。纳米金和放射线均能增加A549细胞的凋亡率，两者联合应用细胞凋亡率显著增加。克隆形成实验表明纳米金对人肺腺癌细胞株A549具有放射增敏作用，初步探讨其机制可能为抑制细胞亚致死损伤的修复，阻滞细胞于G2／M期，并诱导细胞凋亡。关键词：纳米金；A549细胞；放射敏感性；细胞凋亡山东入学硕

3、：学位论文RadiosensitiVityEnhancementofHumanLungAdenocarcinomaCellLineA549bvGlu．GNPsSpeciality：OncologyMasterc

4、andidate：LiXiaohongSupervisorprofessor：Prof．HuLikuanABSTRACTObjective：Radiotherapyholdsanimportantplaceinthecomprehensivetreatmentofmalignancies，anditisinvolvedinmorethan2／3tumorpatients．RadiosensitiVityisthekeytoradiotherapyoftumor．Theuseofradiosensitizercanreduceradiotherapydoseeffectivelyande

5、nhanceradiosensitivityoftumorcells．Thereforeitcanincreaseeffectivenessofradiotherapyandlowerinjurytonormaltissuesandorgans．GoldnanoparticleshavebeenprovedtoenhanceradiosensitivityofmanytumorcellsSOtoimprovetheefficacy,butthereportoflungcancerbygoldnanoparticleswasseldom，andthemechanismwasnotclea

6、r．TostudyradiosensitivityenhancementandthemechanismofhumanlungadenocarcinomacelllineA549bythio—glucoseboundgoldnanoparticles(Glu—GNPs)，andoffernewplatformforthetreatmentoflungcancer．Methods：1．MTTassaywasusedtodeterminetheeffectofgoldnanoparticlesinlowconcentration(<20nmol／L)withorwithout8GyX-ray

7、sonthesurvivalofA549cells．2．TheeffectofradiotherapyenhancementonA549cellsbyGlu—GNPswasmeasuredbyclonalsurvival．3．Thedistributionofcellcycleandthechangeofapoptosisratewereassayedbyflowcytometricmethod(FCM)Results：1．Withdifferentconcentrationsf5、10、15、20nmol／L)ofGlu—GNPsonA549cellsfor48hours．thece

8、llviabilitywas99．1士0．85％、98．1士1．22％、97．5+1．31％and96．8土1．003％respectively．Therewasnoobviousdifferencebetweenthesedata(P>O．05)．Withdifferentconcentrations(O、5、10、15、20nmol／L)ofGlu—GNPsonA549cellsfor24hours，andassociatedwithrad