flavopiridol对eca9706细胞株放射增敏作用的体外研究

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1、carcinomacelllines)[101，andwithstronginhibitoryeffectonthegrowthofhumanJuvesarcomacellline[¨1．While，themostcommonesophagealcancerinChinaissquamouscellcarcinoma，itisnotyetreportedthatwhetherFlavopiridolhaveradiosensitivity—enchancingeffectonEca9706cellline．Therefore，this

2、experimentculturedEta9706cellsinvitro，treatedthecellswithFlavopiridolor／andradiotherapy，testedthecellsurvivalrate，cellcycleandapoptosisrelatedsignalpathways，toinvestigatetheradiosensitivity—enchancingeffectofFlavopiridolonEca9706celllineanditsrelatedmechanism，providenew

3、ideasandmethodsforclinicaltreatmentofesophagealcancers．Purpose：Toinvestigatetheradiosensitivity-enchancingeffectofFlavopiridol(inhibitorsofthecellcycleproteinkinase—CDK)onEca9706cellline(humanesophagussquamouscellline)anditsrelatedmechanism·Methods：Assayedtheinhibitoryr

4、ateofgrowthandradiosensitivity‘enchancingrateofthecelllineunderFlavopiridoleffectbyMTTandcloningexperiment．Usingimmunocyto-chemistrytomeasuretheexpressionofsurvivin，caspase一3，P53．TestedtheapoptosisoftheEca9706celllineindifferentgroupsby玎邛范L．Results：1．ResultsofMTTandclon

5、ingexperimentshowedthatFlavopiridolcouldinhabitthesurvivalrateofEca9706cellssignificantly，relatedwithconcentrationandtime，theIC50was2．561xmol／L．2．ThedifferencesbetweenthesurvivalfractionsofcellstreatedwithFlavopiridolcombinedwithradiotherapyandsingleradiotherapyhadstati

6、sticalsignificance(P<0．05)．Manytargetclickmodel，thesurvivalcurvesfitting：thesensitizationenhancementratioofEca9706celllineR+O。1xlC50，R+0．2xlC50were1．2375and1．3456．3．Resultsofimmunocytochemistryshowedthatthepositiverateofcaspase一3inthetestgroupwas67％，haddifferenceswithth

7、eothergroups(P<0．05)；thepositiverateofsurvivininthetestgroupwas24％，haddifferenceswiththeothergroups(P<0．05)；thepositiverateofP53inthetestgroupwas31％，haddifferenceswiththeothergroups(P<0．05)．4．ResultsofTUNELshowedthatapoptosisindex(AI)ofdruggroupwas(35．8士1．94)％，AIofradia

8、tiongroupwas(47．1+1．71)％，AIofthetestgroupwas(68．3士2．24)％，AIofthecontrolgroupwas(5．14-0．71)％．4Conclusion：1．Flav