资源描述：
《1 Application of flow cytometry in marine phytoplankton research current applications and future perspectives.pdf》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。
1、SCI.MAR.,64(2):121-134SCIENTIAMARINA2000AQUATICFLOWCYTOMETRY:ACHIEVEMENTSANDPROSPECTS.M.RECKERMANNandF.COLIJN(eds.)Applicationofflowcytometryinmarinephytoplanktonresearch:currentapplicationsandfutureperspectives*MARCELJ.W.VELDHUISandGIJSBERTW.KRAAYNetherlandsInstituteforSeaResearch.P.O.Box59
2、,1790ABDenBurg,Texel,TheNetherlands.E-mail:Veldhuis@NIOZ.nlSUMMARY:Abriefoverviewisgivenofcurrentapplicationsofflowcytometry(FCM)inmarinephytoplanktonresearch.Thispaperpresentsaselectionofhighlightsandvarioustechnicalandanalyticalproblemsweencounteredduringthepast10years.Inparticular,theconv
3、ersionoftherelativevaluesobtainedintermsofsizeandfluorescenceapplyingFCMtoquantitativeestimatesofcellsize,pigmentconcentration,genomesizeetc.,isaddressed.TheintroductionofDNA-cell-cycleanalysismadeeasilyassessablebyflowcytometryhasbeenofgreatimportance,allowinginsitumeasurementofspeciesspeci
4、ficgrowthrates.Keyquestionsinecologysuchasfactorsdeterminingthewaxandwaneofphytoplanktonbloomcannowbebetteransweredintermsofspeciesspecificgrowthandmortality.Finally,flowcytometryprovidesdetailedinformationofthephysiologicalstatusoftheindividualalgalcells.Newstainingmethodsenableustodistingu
5、ishbetweenviableandnon-viablecellsandsowillhelpustoelucidatetheimportanceof“automortality”inaquaticecosys-tems.Keywords:autofluorescence,automortality,DNA,flowcytometer,growthrate,phytoplankton,viability.HISTORYTheconfigurationofmostcommerciallyavail-ableinstrumentsisbasedondealingwithpartic
6、lesThefirstandsofarbestknownapplicationofvaryinginsizebetween0.5and30mmandparticleflowcytometry(FCM)inaquaticsciencesisinphy-numbersrangingfrom102to106perml,thuscover-toplanktonresearchoriginallyaimedforametricandingamajorregionofparticlesizedistributionintheataxonomicpurposes(Paauetal.,1978
7、;Yentschetocean(Fig.1).Essentiallytheopticalcharacteristicsal.,1983).Sincetheearly80’sFCMisgenerallyofsingleparticlesinasolutionaremeasuredduringacceptedasananalysingtechnique(seealsoBurkill,theirpassagethroughanarrowbeamoflight(lamp1987;Yentschand
