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1、IntroductiontoFlowCytometry:ALearningGuideManualPartNumber:11-11032-01April,2000BDBiosciences2350QumeDriveSanJose,CA95131-18071-800-448-2347IntroductiontoFlowCytometry:ALearningGuideCopyright©2000Becton,DickinsonandCompany.Allrightsreserved.Nopartofthispublicationma
2、ybereproduced,transmitted,transcribed,storedinretrievalsystems,ortranslatedintoanylanguageorcomputerlanguage,inanyformorbyanymeans:electronic,mechanical,magnetic,optical,chemical,manual,orotherwise,withoutthepriorwrittenpermissionofBDBiosciences,2350QumeDrive,SanJos
3、e,CA95131,UnitedStatesofAmerica.DisclaimerBDBiosciencesreservestherighttochangeitsproductsandservicesatanytimetoincorporatethelatesttechnologicaldevelopments.Thisguideissubjecttochangewithoutnotice.BDBioscienceswelcomescustomerinputoncorrectionsandsuggestionsforimpr
4、ovement.Althoughthisguidehasbeenpreparedwitheveryprecautiontoensureaccuracy,BDBiosciencesassumesnoliabilityforanyerrorsoromissions,norforanydamagesresultingfromtheapplicationoruseofthisinformation.TrademarksApple,theApplelogo,Mac,Macintosh,andPowerMacintosharetradem
5、arksofAppleComputer,Inc.,registeredintheU.S.andothercountries.FinderisatrademarkofAppleComputer,Inc.ModfitLTandQuantiCALCaretrademarksofVeritySoftwareHouse,Inc.CellQuest,FACS,FACSCalibur,FACScan,FACSort,FACStarPLUS,andFACSVantagearetrademarksofBecton,DickinsonandComp
6、any.ContentsPrefaceiiiChapter1:Overview5Chapter2:Fluidics9Chapter3:GenerationofScatterandFluorescence133.1LightScatter..........................................................133.2Fluorescence..........................................................16Chapter4:Opti
7、calSystem194.1OpticalBench........................................................194.2OpticalFilters.........................................................224.3SignalDetection.......................................................244.4Threshold...................
8、.........................................26Chapter5:DataAnalysis275.1DataCollectionandDisplay.............................................
