9 Flow cytometry and integrated imaging.pdf

《9 Flow cytometry and integrated imaging.pdf》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。

1、SCI.MAR.,64(2):247-254SCIENTIAMARINA2000AQUATICFLOWCYTOMETRY:ACHIEVEMENTSANDPROSPECTS.M.RECKERMANNandF.COLIJN(eds.)Flowcytometryandintegratedimaging*V.KACHELandJ.WIETZORREKMax-Planck-InstitutfürBiochemie,D-82152Martinsried,GermanySUMMARY:Itisaseriousproblemtorelatetheresultsofaflowcyto

2、metricanalysisofamarinesampletodifferentspecies.Imagesofparticlesselectivelytriggeredbytheflowcytometricanalysisandpickedoutfromtheflowingstreamgiveavaluableadditionalinformationontheanalyzedorganisms.Thetechnicalprinciplesandproblemsoftriggeredimag-inginflowarediscussed,aswellasthepos

3、itioningoftheparticlesintheplaneoffocus,freezingthemotionofthequick-lymovingobjectsandwhatkindsoflightsourcesaresuitableforpulsedillumination.Theimageshavetobestoredeitherbyfilmorelectronically.Thefeaturesofcameratargetsandthememoryrequirementsforstoringtheimagedataandtheconditionsfort

4、hetriggeringdeviceareshown.Abriefexplanationofthefeaturesofthreerealizedflowcytometricimag-ing(FCI)systemsisgiven:theMacroFlowPlanktometerbuiltwithintheEUROMARMAROPTproject,theImagingMod-uleoftheEuropeanPlanktonAnalysisSystem,supportedbytheMASTIIEurOPAprojectandthemostrecentlydeveloped

5、FLUVOVIuniversalflowcytometerincludingHBO100-andlaserexcitationforfluorescenceandscatter,CoultersizingaswellasbrightfieldandandphasecontrastFCI.Keywords:flowcytometry,imaging,flashillumination,nanolite,LED-flash.INTRODUCTIONistocombineinthesameinstrumenttheabstractflowcytometricanalysi

6、sandtheuptakeofimagesofRelatingtheresultsofflowcytometricmeasure-objectsofparticularinterest.Flowcytometricimag-menttothemoreorlessknowndifferentspeciesining(FCI),however,isahighspeedprocessinvolv-asamplerepresentsaseriousproblem.Iffreewateringproblems,whichcomplicateitsimplementation.

7、samplesareanalyzeditisofgreatinteresttoknowThispapergivesashortintroductionintoprincipleswhatkindofcellsororganismsarehiddenbehindandconstructionofsuchcombinedsystems.theabstractpeaksshownonthescreenoftheflowWestartedwithFCIinvestigatingtechnicalcytometer.Amoreorlesscircumstancialmet