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1、http://www.bdbiosciences.com/research/multicolor/spectrumguide/index.jsp#search=(spectrumview)MulticolorFlowCytometrySpectrumGuideWhenmakingdecisionsaboutwhichfluorochromestouseinyourexperiments,you'llwanttoknowtheirrelativeemissionspectra.Simplymouseoverthespectrumontheleftsi
2、detoviewhistogramsthatrepresenttheabsorptionandemissionspectraforeachBD™fluorochrome.BrilliantViolet™421(Ex-Max407nm/Em-Max421nm)isapolymer-baseddyeexcitedbythevioletlaserandisoneofthebrightestfluorochromesofferedbyBDBiosciences.Conjugatesaretypically10timesbrighterthanPacific
3、Blue™conjugatesandareoftenasbrightasorbrighterthanPEconjugates.Duetonearlyidenticalexcitationandemissionpropertiesbutdifferentspillovercharacteristics,BrilliantViolet421,PacificBlue™,andBDHorizonV450™cannotbeusedsimultaneously.BDHorizon™V450(Ex-Max404nm/Em-Max448nm)isacoumarin
4、dyeexcitedbythevioletlaserthatexhibitsspectralpropertiessimilartoPacificBlue™.ConjugatesaretypicallyasbrightorbrighterthancomparablereagentsconjugatedtoPacificBlue™.Duetonearlyidenticalexcitationandemissionpropertiesbutdifferentspillovercharacteristics,BrilliantViolet421,Pacif
5、icBlue™,andBDHorizonV450cannotbeusedsimultaneously.PacificBlue™(Ex-Max401nm/Em-Max452nm)isbasedonthe6,8-difluoro-7-hydroxycoumarinfluorophore,andisstronglyfluorescent,evenatneutralpH.Duetonearlyidenticalexcitationandemissionpropertiesbutdifferentspillovercharacteristics,Brilli
6、antViolet421,PacificBlue™,andBDHorizonV450cannotbeusedsimultaneously.AmCyan(Ex-Max457nm/Em-Max491nm)isa108-kDaproteinderivedfromAnemoniamajano.Withanexcitationpeakof458nmandanemissionpeakof491nm,itcanbeusedonvioletlaser–equippedflowcytometersincombinationwithBrilliantViolet421
7、,BDHorizonV450,orPacificBlue™,butnotBDHorizonV500.BDHorizon™V500(Ex-Max415nm/Em-Max500nm)isanovelorganicdyeexcitedbythevioletlaser.ThisdyeoffersimprovedbrightnessoverPacificOrange™andreducedspilloverintotheFITCchannelwhencomparedtoAmCyan.BDHorizonV500cannotbeusedsimultaneously
8、withAmCyanorPacificOrange™.AlexaFluor®488(Ex-Max495nm/Em-Max5
