鱼藤酮对pc12细胞caspase_3_fas和fasl的作用及与凋亡的关系

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1、1888第四军医大学学报(JFourthMilMedUniv)2005;26(20)http://journal.fmmu.edu.cn·研究原著·文章编号:100022790(2005)2021888204鱼藤酮对PC12细胞Caspase23,Fas和Fasl的作用及与凋亡的关系赵黔鲁,柴锡庆(河北医科大学研究生学院,河北石家庄050017)EffectofrotenoneonCaspase23,Fasuncertainly,andincreasedpoikilochromatincollectedinedge.CONCLUSION:Rotenonemay

2、damagethePC12andFaslandrelationshipwithapopto2cellsbyactivationofCaspase23andFas/FaslapoptosissisinPC12cellspathways.ZHAOQian2Lu,CHAIXi2Qing【Keywords】Rotenone;apoptosis;Parkinsondisease;mi2GraduateCollegeofHebeiMedicalUniversity,Shijiazhuangcroscopy,electron050017,China【摘要】目的:探讨鱼藤酮对

3、PC12细胞Caspase23,Fas和【Abstract】AIM:ToinvestigatetheeffectofrotenoneonFasl的作用及与凋亡的关系.方法:应用流式细胞仪(flowcy2Caspase23,FasandFaslandrelationshipwithapoptosisintometry,FCM)检测不同浓度鱼藤酮对体外培养大鼠肾上腺PC12cells.METHODS:PC12cellsweretreatedwithdiffer2嗜铬细胞瘤(PC12)细胞株凋亡率的影响,并检测1.0μmol/Lentconcentrationsof

4、rotenoneandtheeffectofrotenoneon鱼藤酮干预不同时段对Caspase23、Fas/Fasl蛋白表达的影响,theratioofapoptosiswastestedbyflowcytometry.1.0透射电镜观察凋亡形态学的改变.结果:对照组凋亡率为μmol/LrotenonewasaddedintoPC12cellsculturemedium0.0199±0.0020,经0.1,1.0,2.0和3.0μmol/L不同浓度鱼atdifferenttimesandtheproteinexpressionofCaspase23藤酮干预

5、2d,凋亡率明显增加其变换值分别为:0.0305±andFas/FaslwasalsotestedusingFCM.Themorphologi20.0030,0.0486±0.0073,0.0313±0.0041和0.0285±calchangesofapoptosiswereobservedundertransmitting0.0031,与对照组比较有显著差别(P<0.02),实验组中以1.0electronmicroscopy(TEM).RESULTS:Theratioofapop2μmol/L作用最显著(P<0.01),当鱼藤酮浓度大于1.0μmol/t

6、osisincontrolgroupwas0.0199±0.0020.InthetrialL时,其作用呈剂量依赖性递减.对照组Caspase23,Fas和groupstreatedwithdifferentdosesofrotenonefor2d,theFaslFI值分别为1.0±0.03,1.0±0.02和1.0±0.04;经鱼藤ratioincreasedas0.0305±0.0030,0.0486±0.0073,0.0313酮干预3,6,12,24和48h,各组均有Caspase23,Fas和Fasl±0.0041and0.0285±0.0031,res

7、pectively,significantly表达,其中以鱼藤酮干预6h时Fas/Fasl表达最明显(P

8、论:鱼藤creasedinadose2