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《关键词α-l-岩藻糖苷酶(afu)甲胎蛋白(afp)胸苷激酶1(tk1)原发性肝癌联合检测》由会员上传分享，免费在线阅读，更多相关内容在行业资料-天天文库。
1、ROC曲线及Logistic回归评价血清AFU、AFP和TK1在原发性肝癌的诊断价值张诗颜作者简介：张诗颜，男，1974年生，硕士，副主任技师，主要从事临床免疫学工作。通讯作者：张诗颜，联系电话：0593-7832030，Email:myebox@139.com.，李步任（福建中医药大学附属福鼎医院检验科福建福鼎355200）摘要：目的探讨血清α-L-岩藻糖苷酶(α-L-fucosidase,AFU)、甲胎蛋白(alpha-fetoprotein,AFP)和胸苷激酶1（ThymidineKinase1,TK1）在原发性肝癌（Primary
2、LiverCancer，PLC）诊断中的临床意义。方法测定116例PLC患者、109例良性肝病患者血清AFU、AFP和TK1水平，并与104例健康正常对照的血清浓度水平进行比较。用Logistic回归模型绘制ＲOC曲线并计算曲线下面积(AUCＲOC)以评价各指标的诊断价值。结果PLC组和良性肝病组患者比较，PLC组血清AFP和TK1水平高于良性肝病组(Z=-8.570，-5.943，P均<0.001)，差异有显著统计学意义，而此两组中AFU比较，差异无统计学意义（Z=-1.820,P=0.069）。良性肝病组AFU高于正常对照组，差异有显
3、著统计学意义（Z=-7.984，P<0.001）。PLC组与非肝癌组诊断性能评价ROC曲线下最佳cut-off值：AFU为40.80U/L，AFP为10.86μg/L和TK1为1.92pmol/L；ROC曲线下的面积AFU为0.718，AFP为0.832，TK1为0.773，预测概率值pre-1的曲线下面积为0.900，其约登指数（68.4%）和敏感度（85.3%）最高。结论血清AFU、AFP和TK1联合检测对PLC的诊断具有重要价值,并且可显著增加PLC诊断的约登指数和敏感性。关键词：α-L-岩藻糖苷酶(AFU):甲胎蛋白(AFP):胸
4、苷激酶1（TK1）:原发性肝癌:联合检测EvaluationoftheDiagnosticValueofSerumAFU,AFPandTK1forPrimaryLiverCancerwithLogisticRegressionandROCCurveZHANGShiyan,LIBurenDepartmentofClinicalLaboratoryofAffiliatedFudingHospital,FujianUniversityofTraditionalChineseMedicine,FujianFuding355200,ChinaAbs
5、tract：ObjectiveTostudytheclinicalsignificanceofdetectionofserumα-L-fucosidase(AFU),alpha-fetoprotein(AFP)andthymidinekinase1(TK1)indiagnosingprimarylivercancer(PLC).MethodsSerumlevelsofAFU,AFPandTK1weremeasuredin116patientswithprimarylivercancer,109patientswithbenignhepat
6、icdiseasesand104normalsubjects.Thediagnosticvaluewasanalyzedbylogisticregressionequationandreceiveroperatingcharacteristiccurves(ＲOC).ResultsTheserumlevelsofAFPandTK1inpatientswithPLCweresignificantlyhigherthanthoseinpatientswithbenignhepaticdiseases(Z=-8.570,-5.943.allP<
7、0.001).However,therewasnostatisticallysignificantdifferenceofAFUbetweenthesetwogroups(Z=-1.820,P=0.069).ThelevelsofAFUweresignificantlyhigherinpatientswithbenignhepaticdiseasesthanthoseinthenormalsubjects(Z=-7.984,P<0.001).Receiveroperatingcharacteristiccurves(ROC)inpatie
8、ntswithPLCsversusthosewithoutPLCsindicatedtheoptimalcut-offvaluewas40.80U/LforAFU,10.86μg/LforAF
