shrna-hiap2增强cne-1鼻咽癌细胞对放射敏感性的实验分析

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1、万方数据forenhancedradiosensitivityoftheinitialinvestigatethemechanismtheadjuvanttreatmentofelinicalgene1ayacertainfoundation．Abstract：Objective：ConStructtheinhibitorofthes删Aofapoptosisprotein(姒P一2)forthepurposeofthecombinationofinhibitionhIAP一2genewithradiotherapy,in

2、vestigateitseffecttowardsCNE1nasopharyngealcarcinomacelllineradiosensitivity．Methods：First，usingtheMTTassaytodeterminetheoptimaldoseofradiation；ThenbuildfourhIAP．2一S删AsilencingfragmentandtransfectedbyliposomeCNE1cells．andfinallyusereal．timequantitativePCR(Q—PCR)an

3、dWesternblot(WB，Westernblot)bestsilencingshRNAsequencesscreened；UsingsilenceshRNAsequencesbesttransfectionCNE1cells，respectivelysetuptransfectedirradiatedgroupandnon··transfectedirradiatedgroup；usingreal--timequantitativePCRandWBdetect11IAP-2geneandproteinexpressi

4、on，usingimmunocytochemistrydetecthIAP一2proteinexpression，apoptosisdetectesbyflowcytometry．transwellmeasurescellinvasion．Results：MTTassaydetermined4Gyastheoptimalradiationdose；O．PCRandWBtestresultsshowedthatfourhIAP．2．ShI之NAcouldeffectivelyinhibit11IAP．2mRNAandprot

5、einexpression．whichhiAP一2-shRNA2silencehasthemostsignificanteffect；Q-PCR，WBandimmunocytochemistrytestresultsshowedthathL～P．2geneandproteinexpressionofthetransfecfionhiAP．2．S删A2irradiatedgroupanduntransfectedhiAP．2．ShRNA2C卜m1cellsirradiatedgroupweresignificantlydif

6、ferent；FlowcytometryshowedthattransfectionhIAP．2．SbRNA2irradiatedgroupthaninthenon．transfectedhIAP．2．S㈣A2irradiationapoptosisrateweresignificantlydifferent：TranswelltestresultsshowedthattranSfectionhiAP．2．S删A2irradiatedgroupanduntransfected瑚-AP一2一shRNA2invasionirr

7、adiatedgroupweresignificantlydifferent；Conclusion：TranslfectionhIAP．2genesilencingafterradiotherapyCanincreasenasopharyngealcarcinomacellapoptosisrate，reducingitsinvasion，Canplayaroleinradiosensitization．Keywords：hIAP一2，Nasopharyngealcarcinomacells，RNAsilencing，Ra

8、diosensitivityV万方数据目录原创性声明⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯I中文摘要⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯II英文摘要⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯．．IV目录⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯