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1、ClinicalStudyonEffectofErzhiTianguiGranuleinImprovingGrowthDifferentiationFactor-9(GDF-9)mRNAExpressionLevelsintheGranulosaCellsandtheQualityofOocytefromPatientsSpeciality:GynecologyofTCM,theStudyofFemaleSterilityAuthor:MinminWeiTutor:Prof.FangLianAbstractObjective:Toinvesti
2、gatetheeffectsofErzhiTianguiGranuleonthekidneyinpatientsundergoinginvitrofertilization-embryotransfer(IVF-ET)cycleofgrowthdifferentiationfactor-9(GDF-9)expressionlevelsingranulosecells.Methods:Sixty-sixtubalinfertilitypatientsundergoingIVF-ETwererandomlyassignedtotwogroups,T
3、hirty-threepatientsinthetreatmentgroupweretreatedwithErzhiTianguiGranuleandGnRHa/FSH/hCG,andtheotherthity-threepatientsinthecontrolgroupweretreatedwithPlaceboandGnRHa/FSH/hCG.Toobservetheclinicalbasicsituationoftwogroupsofpatientsbeforetreatment,theclinicalsymptomsofintegral
4、comparisonbeforeandafterthetreatmenttwogroups,toobservethecomparisonofbasedendocrineFSH,E2beforetreatmenttwosetsofpatients.Afterthetreatment,Real-timePCRwasperformedtodetectthelevelsofGDF-9mRNAingranulosecellsobtainedfromdifferentresponsegroups.Thedoseofgonadotropin(Gn),then
5、umberofoocytes,theratesoffertilization,oocytecleavage,highqualityoocyte,highqualityembryo,andpregnancywerecompared,totesttheCorrelationbetweenthelevelsofGDF-9mRNAingranulosecellsandtherateofhighqualityoocyteaswellastherateofhighqualityembryo.Results:(1)Beforetreatmenttwogrou
6、pswerecomparableandtherewasnoignificantdifference(P>0.05).AtthesameTime,therewerenosignificantdifferencesformbased2endocrineFSH,E2,AFCresultsbetweentwogroupsofpatients(P>0.05),itwascomparable.(2)Clinicalsymptomsimprovedmuchintreatmentgroupthancontrolgroup(p<0.05),andtherewer
7、esignificantdifferences.(3)TherewasnosignificantdifferenceinthedoseofGnorthenumberofoocytesaswellastherateofoocytecleavage.(4)Theratesoffertilization,highqualityoocyte,highqualityembryo,andpregnancyweresignificantlyhigherinthetreatmentgroupthaninthecontrolgroup(p<0.05).(5)Th
8、eexpressionofGDF-9mRNAinthegranulosacellsweresignificantlyhigherinthetreatm