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1、ClinicalStudyonEffectofErzhiTianguiGranuleinImprovingGrowthDifferentiationFactor-9（GDF-9）mRNAExpressionLevelsintheGranulosaCellsandtheQualityofOocytefromPatientsSpeciality:GynecologyofTCM，theStudyofFemaleSterilityAuthor:MinminWeiTutor:Prof．FangLianAbstractObjective:Toinvesti
2、gatetheeffectsofErzhiTianguiGranuleonthekidneyinpatientsundergoinginvitrofertilization-embryotransfer(IVF-ET)cycleofgrowthdifferentiationfactor-9（GDF-9）expressionlevelsingranulosecells.Methods:Sixty-sixtubalinfertilitypatientsundergoingIVF-ETwererandomlyassignedtotwogroups,T
3、hirty-threepatientsinthetreatmentgroupweretreatedwithErzhiTianguiGranuleandGnRHa/FSH/hCG,andtheotherthity-threepatientsinthecontrolgroupweretreatedwithPlaceboandGnRHa/FSH/hCG.Toobservetheclinicalbasicsituationoftwogroupsofpatientsbeforetreatment,theclinicalsymptomsofintegral
4、comparisonbeforeandafterthetreatmenttwogroups,toobservethecomparisonofbasedendocrineFSH,E2beforetreatmenttwosetsofpatients.Afterthetreatment，Real-timePCRwasperformedtodetectthelevelsofGDF-9mRNAingranulosecellsobtainedfromdifferentresponsegroups.Thedoseofgonadotropin(Gn),then
5、umberofoocytes,theratesoffertilization,oocytecleavage,highqualityoocyte,highqualityembryo,andpregnancywerecompared,totesttheCorrelationbetweenthelevelsofGDF-9mRNAingranulosecellsandtherateofhighqualityoocyteaswellastherateofhighqualityembryo.Results:(1)Beforetreatmenttwogrou
6、pswerecomparableandtherewasnoignificantdifference(P>0.05).AtthesameTime，therewerenosignificantdifferencesformbased2endocrineFSH,E2，AFCresultsbetweentwogroupsofpatients(P>0.05),itwascomparable.(2)Clinicalsymptomsimprovedmuchintreatmentgroupthancontrolgroup(p<0.05)，andtherewer
7、esignificantdifferences.（3）TherewasnosignificantdifferenceinthedoseofGnorthenumberofoocytesaswellastherateofoocytecleavage.（4）Theratesoffertilization,highqualityoocyte,highqualityembryo,andpregnancyweresignificantlyhigherinthetreatmentgroupthaninthecontrolgroup(p<0.05).（5）Th
8、eexpressionofGDF-9mRNAinthegranulosacellsweresignificantlyhigherinthetreatm
