神经生长因子对大鼠脑缺血再灌注后tgf-β_2c1_和il-1β表达的影响

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1、Effectofnervegrowthfactoronexpressionoftransforminggrowthfactor—PlandInterleukin—lBaftercerebralischemicalreperfusionPostgraduate：ZhangHongmeiSupervisor：ProfessorXinshimengSpecialty：NeurologyAbstractObjective：applyingfocalischemia·reperfusionmodelofSDrat，t

2、oobservethechangeofexpressionofInterleukin·113(IL-l13)andtransforminggrowthfactor．pl(TGF—p1)andthenervegrowthfactoroneverytimeratsafterfocalcerebralischemicalreperfusioninjuryinSprague’Dawley(SD)rat．toinvestigatethebrainprotectingmechanismofnervegrowthfact

3、orandtoofferthetheoryofcuringischemiccerebrovasculardisease·Method：55healthyadultSDrats，femaleandmaleunlimited，theweight200．2509，sweepclass，feedenvironmenttemperature21℃，takeamealwaterfreely．Theywererandomlydividedintoafalsesurgicaloperationgroup(n=5)(Grou

4、pl)；Matchedgroup(n230)，accordingtoischemic2hreperfusion2h，6h，12h，24h，48h，72hisdividedinto6sets(respectively1S2、3、4、5、6and7groups),each5；ThenervegrowthfactorgrouPfn；10)(Group8)andmatchedgroup(n=10)(Group9)inphysiologybrine·AccordingtoNagasawareport，toanaest

5、hetizeanimalwith10％chloralhvdrate(0．35ml／1009bellycavitiesesinject)，taketolieonthebackonefixed．gorightsideMCAOsurgicaloperationinthesurgicaloperationonthestage，theneckslicesexactcenter，separatingarightsidecommoncarotidartery，externalcarotidarteryandinterna

6、lcarotidartery，ligaturethecarotidarterynearheartandexternalcarotidarterybranchstart，slicetoshear1inthecarotidarterybranchstart，headcarrytodiptohaVeaPurepaintofdiameterforO．20mmfishingthreadcarriestoinserttowardinternaIcarotidartery，continuingtopushforwardu

7、ntilfeelslightresistance，thenabout18millimeters，hinttohavealreadygottobraininmiddlecerebralartervstartdepartment．After2hours，slowlypromotethefishingthreadthentoimplementreperfusion．Aftertheanimalrevivals，observeitspostureandbehavior，judgetheMCAOmodeltobesu

8、ccessful．Afterthefalsesurgerygroupinsertsthenylonlinetoextractimmediately，oldstepwithsurgerygroup．Aftertheischemiareperfusionmodelanimalstobesober，theleftforelimbflexureadductionratistheresearchobject．The8thg