神经生长因子对大鼠脑缺血再灌注后caspase-3与angⅱ表达的影响

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1、Effectofnervegrowthfactoronexpressionofcaspase一3andAnglIinrataftercerelpralischeidcaiperfusionCerebraliscItemlcairepeusnMasterdegreecandidate：ZhangyuSupervisor：ProfessorXinshimengMajor：NeurologyAbstractObjective：Applyingfocalischcnda-repcffusionmodelofSDrat,toobservethechangeofexpressionofc

2、aspase-3andAngnortheeffectofnervegrowthfactoroneverytimeratsafterfocalcerebralischemicalreperfusioninjuryinSpraguc-Dawley(SD)rat,toinvestigatethecerebralischemicalreperfusionprotectingmeehanisrnofnervegrowthfactor(NGF)andtoofferthetheoryofcuringischemiccerebrovaseulardisease．Method：45health

3、yadultSDrats，femaleandmaleunlimited，theweight200"--2509，sweepclass，feedenvironmenttemperature21℃，takeamealwaterfreely．Theywererandomlydividedintoafalsesurgicaloperationgroup(n=5)(Group1)；matchedgroup(n230)，accordingtoischemic2hreperfusion2h，6h，12h，24h，48h，72hisdividedinto6sets(respectiVelyi

4、s2,3，4，5，6and7groups)，each5；thenervegrowthfactorgroup(n=5)(Group8)andmatchedgroup(n=5)(Group9)inphysiologicalsaline．AccordingtoNagasawareport，toanaesthetizeanimalwith10％chloralhydrate(0．35ml／1009bellycavitiesesinject)，taketolieonthebackonefixed，gorightsideMCAOsurgicaloperationinthesurgicalo

5、perationonthestage，theneckslicesexactcenter,separatingafightsidecommoncarotidartery,externalcarotidarteryandinternalcarotidartery，ligaturethecarotidarterynearheartandexternalcarotidarterybranchstart，slicetoshearoneinthecarotidarterybranchstart，headcarrytodiptohaveapurepaintofdiameterforO．20

6、mmfishingthreadcarriestoinserttowardinternalcarotidartery,continuingtopushforwarduntilfeelslightresistance，thenabout18ramtohavealreadygottobraininmiddlecerebralarterystartdepartment．After2hslowlypromotethefishingthreadthentoimplementreperfusion．Aftertheanimalrevivalsobservesitspostureandthe

7、behaviorjudgestheMCAOmodeltobesuccessful．Afterthefalsesurgerygroupinsertsthenylonlinetoextractimmediately,oddstepwithsurgery3group．Aftertheischemia—reperfusionmodelanimalstobesober，therightforelimbflexureadductionratistheresearchobject．The89roupofratsaft