神经生长因子对大鼠脑缺血再灌注后fas-l蛋白和p-pkb表达的影响

《神经生长因子对大鼠脑缺血再灌注后fas-l蛋白和p-pkb表达的影响》由会员上传分享，免费在线阅读，更多相关内容在行业资料-天天文库。

1、EffectofNerveGrowthFactoronExpressionofp-PKBAndFas-LGeneProteininRatAfterCerebrallschemialReperfusionMaserdegreecandidate：LiuPengSupervisorProfessor：ProfessorXinShimengMajor：NeurologyAbstractObjective：AdoptingfocalcerebralischemiaandreperfusionmodelofSpra

2、gue-Dawleyrat(SDrat)toobserveinjuryoftheneuralfunction，expressionofP—PKB(phosphoryla—tionofProteinKinaseB)andFas·LgeneproteinoneverytimeafterreperfusionoflocalcerebralischemiaandtoobservetheprotectivefunotionofNGFinthebrainofratandfurthertoinvestigatetheb

3、rainprotectingmechanismofNGFandtoofferthetheoreticalbasisforclinicaltherapyofischemiacerebrovasculardisease．Method：55healthyadultSDratsunlimitedsextakethemealandwaterfreelyandatthesametimetheymustbeprovidedwithfollowcondition：theirweightcontroledbetween20

4、0-2509，cleanlyclass，environmenttemperatureof21centigradedegree．Theywererandonlydividedintofalsesurgicaloperationgroup(n一5)(Group1)，matchedgroups(n230)whichaccordingtoisehemictimeisdividedinto2hgroup，6hgroup，12hgroup，24hgroup，48hgroup，72hgroup(5ratsineachg

5、roup)，theNGFgroupswhichinclud8fgroup【afterreperfusing6hinjectingNGF)and8pgroup(afterreperfusing72hinjectingNGF)(5SDratsineachgroup)，salinegroupswhichinclude9fgroup(afterreperfusing6hinjectingsaline)and9pgroup(afterreperfusing72hinjeetingsaline)(respectiVe

6、ly5SDrats)．AccordingtoNagasawareport，toanaesthetizeanimalwuth10％chloralhydrate(0．35ml／1009bellycavitiesesinject)，taketolieonthebackonthestage，theneckslicesexactcenter，separatingarightsidecommoncarotidarterynearheartandexternalcaroidarterybranchstart，slice

7、toshear1inthecarotidarterybranchstart，headcarrytodiptohaveapurepaintofdiameterfor0．20mmfishingthreadcarriestolnserttowardInternalcarotidartery，continuingtopushforwardUntilfeelslightresistance，thenabout18millimeters，hinttohavealreadygottobraininmiddlecereb

8、ralarterystartdepartment．After2hoursslowlypromotethefishingthreadthentoinplementreperfusion．AftertheanimalrevivalsobservesitspostureandthebehaviortojudgetheMCAOmodleweatheritissuccessful．Afterthefalsesergerygroupins