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ID:33213109
大小:16.88 MB
页数:26页
时间:2019-02-22
《神经生长因子对大鼠脑缺血再灌注后fas-l蛋白和p-pkb表达的影响》由会员上传分享,免费在线阅读,更多相关内容在行业资料-天天文库。
1、EffectofNerveGrowthFactoronExpressionofp-PKBAndFas-LGeneProteininRatAfterCerebrallschemialReperfusionMaserdegreecandidate:LiuPengSupervisorProfessor:ProfessorXinShimengMajor:NeurologyAbstractObjective:AdoptingfocalcerebralischemiaandreperfusionmodelofSpra
2、gue-Dawleyrat(SDrat)toobserveinjuryoftheneuralfunction,expressionofP—PKB(phosphoryla—tionofProteinKinaseB)andFas·LgeneproteinoneverytimeafterreperfusionoflocalcerebralischemiaandtoobservetheprotectivefunotionofNGFinthebrainofratandfurthertoinvestigatetheb
3、rainprotectingmechanismofNGFandtoofferthetheoreticalbasisforclinicaltherapyofischemiacerebrovasculardisease.Method:55healthyadultSDratsunlimitedsextakethemealandwaterfreelyandatthesametimetheymustbeprovidedwithfollowcondition:theirweightcontroledbetween20
4、0-2509,cleanlyclass,environmenttemperatureof21centigradedegree.Theywererandonlydividedintofalsesurgicaloperationgroup(n一5)(Group1),matchedgroups(n230)whichaccordingtoisehemictimeisdividedinto2hgroup,6hgroup,12hgroup,24hgroup,48hgroup,72hgroup(5ratsineachg
5、roup),theNGFgroupswhichinclud8fgroup【afterreperfusing6hinjectingNGF)and8pgroup(afterreperfusing72hinjectingNGF)(5SDratsineachgroup),salinegroupswhichinclude9fgroup(afterreperfusing6hinjectingsaline)and9pgroup(afterreperfusing72hinjeetingsaline)(respectiVe
6、ly5SDrats).AccordingtoNagasawareport,toanaesthetizeanimalwuth10%chloralhydrate(0.35ml/1009bellycavitiesesinject),taketolieonthebackonthestage,theneckslicesexactcenter,separatingarightsidecommoncarotidarterynearheartandexternalcaroidarterybranchstart,slice
7、toshear1inthecarotidarterybranchstart,headcarrytodiptohaveapurepaintofdiameterfor0.20mmfishingthreadcarriestolnserttowardInternalcarotidartery,continuingtopushforwardUntilfeelslightresistance,thenabout18millimeters,hinttohavealreadygottobraininmiddlecereb
8、ralarterystartdepartment.After2hoursslowlypromotethefishingthreadthentoinplementreperfusion.AftertheanimalrevivalsobservesitspostureandthebehaviortojudgetheMCAOmodleweatheritissuccessful.Afterthefalsesergerygroupins
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