大鼠局灶脑缺血再灌注后内皮素-1表达与神经生长因子脑保护的分析

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1、groupswereinjectedsalinewithequalvolume．Usingamodelofoccludingthemiddlecerebralarteryandreperfusingbythesuturemethodinrats．Allanimalsincontrolgroupweredrawnthestringafterlmin．Animalsinexperimentandtreatmentweredrawnthestringafter90minandreperfused，andobservedtheresultofocclusionandreperfu

2、sion,thentocountsuccessrateanddeathrate．At90minafterischemia,thestringWaSpulledcarefullyandreperfused．At111,6h,24hafterreperfilsion,weevaluatedtheneurologicimpairmentrespectivelyinthelightofZea-Longa’Sstandardsoffivegrades．24hafterreperfusion2animalsincontrolgroup4animalsinexperimentgroup

3、andtreatmentgrouprespectivelystainedwith2％TriphenylTetrazaliumChloride(TTC)for30rain．TojudgetheischemialocationandmeasureinfarctionvolumeandobservethenumberofnecroticnervebyHematoxylin-cosintim)staining．At1k6h,24h,48ll'96hafterischemiaandreperfusion,braintissuewasfixedby4％formaldehydumand

4、CUtintoslicesinordertOdeterminetheexpressionofET·1inischemiasection．ObservedthenecroticnervebyimmunohistochemistryaccordingtOthedifferenttimepoints，andobservedtheeffectofNGFontheaboveindexes．Results：ItWasabout30mintomakemodel．56anim【alswereoccludedsuccessfullythesuooessrateofmodelocclusio

5、nwas80．00％ThesuccessrateofreperfusionWas77．14％and54animalsweresuccessful．AJIanimalsinSham-operatedgroupsurvived；7animalsdiedintheexperimentwhosedeathratewas20％，4animalsdiedinthetreatmentwhosedeathratewasl1．43％．Therew∞statisticallysignificantdifference(p<0．05)．Animalsshowedthene啊olo#cimpai

6、rumntsymptominthesuccessfulmodels．TheIlighestscorewasat24h．R’Sclearthatwiththeprolongationoftime,thesymptomsareaggravatedprogressively．ComparedthetreaUnentgroupwiththeexperimentgroup，thenourologicimlmirmzatsymptomisimprovedgraduallyandthescoreSdecreasedobviously(p(o．05)．24hTTCstainingaRer

7、repeffusion,thecerebralcortexinfarctionintheischemiasidewaspale,theothersideandnoninfarctionWasred．Theischemiasectionsmainlyexsitedinthefrontallobe，parietallobeofcerebralcortexandcaudaten11．AnimalshadnoinfarctioninSham-operatedgroup．Theinfarctvolumewas29．56±3．90％ine