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ID:33755733
大小:5.39 MB
页数:22页
时间:2019-02-28
《神经生长因子对大鼠脑缺血再灌注后tgf-β_2c1_和il-1β表达的影响》由会员上传分享,免费在线阅读,更多相关内容在行业资料-天天文库。
1、Effectofnervegrowthfactoronexpressionoftransforminggrowthfactor—PlandInterleukin—lBaftercerebralischemicalreperfusionPostgraduate:ZhangHongmeiSupervisor:ProfessorXinshimengSpecialty:NeurologyAbstractObjective:applyingfocalischemia·reperfusionmodelofSDrat,t
2、oobservethechangeofexpressionofInterleukin·113(IL-l13)andtransforminggrowthfactor.pl(TGF—p1)andthenervegrowthfactoroneverytimeratsafterfocalcerebralischemicalreperfusioninjuryinSprague’Dawley(SD)rat.toinvestigatethebrainprotectingmechanismofnervegrowthfact
3、orandtoofferthetheoryofcuringischemiccerebrovasculardisease·Method:55healthyadultSDrats,femaleandmaleunlimited,theweight200.2509,sweepclass,feedenvironmenttemperature21℃,takeamealwaterfreely.Theywererandomlydividedintoafalsesurgicaloperationgroup(n=5)(Grou
4、pl);Matchedgroup(n230),accordingtoischemic2hreperfusion2h,6h,12h,24h,48h,72hisdividedinto6sets(respectively1S2、3、4、5、6and7groups),each5;ThenervegrowthfactorgrouPfn;10)(Group8)andmatchedgroup(n=10)(Group9)inphysiologybrine·AccordingtoNagasawareport,toanaest
5、hetizeanimalwith10%chloralhvdrate(0.35ml/1009bellycavitiesesinject),taketolieonthebackonefixed.gorightsideMCAOsurgicaloperationinthesurgicaloperationonthestage,theneckslicesexactcenter,separatingarightsidecommoncarotidartery,externalcarotidarteryandinterna
6、lcarotidartery,ligaturethecarotidarterynearheartandexternalcarotidarterybranchstart,slicetoshear1inthecarotidarterybranchstart,headcarrytodiptohaVeaPurepaintofdiameterforO.20mmfishingthreadcarriestoinserttowardinternaIcarotidartery,continuingtopushforwardu
7、ntilfeelslightresistance,thenabout18millimeters,hinttohavealreadygottobraininmiddlecerebralartervstartdepartment.After2hours,slowlypromotethefishingthreadthentoimplementreperfusion.Aftertheanimalrevivals,observeitspostureandbehavior,judgetheMCAOmodeltobesu
8、ccessful.Afterthefalsesurgerygroupinsertsthenylonlinetoextractimmediately,oldstepwithsurgerygroup.Aftertheischemiareperfusionmodelanimalstobesober,theleftforelimbflexureadductionratistheresearchobject.The8thg
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