头部浅低温对大鼠全脑缺血再灌注损伤及mtor通路的影响

《头部浅低温对大鼠全脑缺血再灌注损伤及mtor通路的影响》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。

1、万方数据英文摘要thatthetemperatureoftheheadcouldbedecreased．Intheprocessoflowingtemperature，ansuctionapparatuswasusedtopreventaspiration．WhenthetemperatureofhippocampusWasreducedto(33．0-t0．5)℃，thebilateralcommoncarotidarterieswereclippedfor15minutes．ThelOWtemperatureWaslastedfor1hourand

2、thenrewarmednaturally．GroupRandgroupDweremedicatedgroups．1hbeforeglobalcerebralischemia,astereotaxicinstrumentwasusedtoslowlyinject5}tlRapamycin(10mmol／1)intoaleftventricleofratsofgroupRandthesRmevolumeofDMSOwasgiventogroupD．TheelectrodeWasusedtopiercethescalptosuperviseforEEC‘t

3、opiercetheskinoflimbstomonitorECGAtemperatureprobewasplacedintherighthippocampalCA1region(anteriorfontanelle3．6mnl，themidlinerightside3millandsuncortical2mm)tomonitorthetemperatureofhippocampus．TherectaltemperatureWassupervisedallthroughthetest．4Samplepreparationanddetection4．1I

4、mmunohistochemicalmethodwasusedtomeasuretheexpressionofP70S6Kprotein，P-P70S6Kproteininbraintissuesandtodeterminatethehematoxylin-eosin(HE)stainingandlightmicroscopydetection．Afterobservedfor2hoursingroupSandreperfusedfor2hoursinotherfourgroups，sixratswererandomlychosetobeanesthe

5、tized．Thenanimalswereperfusedby4％paraformaldehyde(PFA)tofixtissues，afterthisprocedure，thebraintissueswerefleetlygotoutandputin4％PFAforfixation．Sampleswerekeptinfridgeat4℃fornextdetermination．4．2ThemethodofWesternBlotwasusedtodeterminemTORprotein，P．mTORproteininthehippocampus．The

6、othersixratsineachgroupwereanesthetizedby10％chloralhydrate(400mg／kg)andthenkilledwithbrainstissueswerequicklytakenout．ThehippocampaltissueswereCarefullyseparatedandputintoliquidnitrogentopreserveandthenstoredat-80。C．ThesesampleswereusedtomonitortheexpressionofmTORproteinandP-mTO

7、Rprotein．．Results：1Thebodyweightoftheratsinanyofthegroupshasnostatistical6万方数据关文摘要———————————————————————————————————二——二————————一一difference(P>0．05)．2Theresultsoflightmicroscope．GroupS：InthehippocampalCA1regionthemorphologyandstructureofplasmaandappearedred，cellnucleuswerebigan

8、dwereroundnessorovalandtheyshowedclearboundary．