pten活性的抑制联合头部浅低温对大鼠全脑缺血再灌注损伤的影响

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1、中文摘要氧关键词：基因，P吒N；低温，人工；再灌注损伤；脑；凋亡；活性英文摘要Ef．fectsofCombinationofInhibitionofPTENActiVi坶andMildHead删哪Ilia伽G10MCe础跏妯锄脚响sionIIliuIlvillI№mlUIlVmKa娼■，．，ABSTRACTObjective：Amodelofglobalcerebralischemia—repemsionwasestablishedbyusingamethodofmodi矗edPulsinelli’sfour-vesselocclus

2、ioninrat，bpv(pic)wasadministeredtoinhibitPTENactiVitybeforeglobalcerebralischemiaandnasopha叫ngealcoolingwas印pliedtoreduceheadtemperatureinrats．Thepu叩oseofstudywastodetecttheexpressionofP-PTENprotein，Baxprotein，Bcl一2proteininhippoc锄palCA1region，todetermineconcentrationof

3、S1OOBprotein，MDAcontentandSODactiVityinbraintissue．Thee腩ctsofinhibitionofPTENactivity’mildheadhypothemiaandtheircombinationonglobalcerebralischemia—reperfusioninju拶inratswereinVestigated．Methods：SixtyhealthymaleWistarratsweighing250～2809wereusedinthisstudyandrandomlydiV

4、idedintofiVegroups(n212each)：Sh锄operation铲oup(groupS)；Globalcerebralischemia—reper如sioninju巧group(groupIR)；InhibitionofP1ENactiVitygroup(groupB)；Mildhypothermiagroup(groupMH)；CombinationofinhibitionofPTENactiVityandmildhypothemiagroup(伊oupMB)．Allratswereanaesthetizedwit

5、hintraperitoneal1O％chloralhydrate(350mg／kg)．ThebilateralveIrtebralarterieswereelectrocauterized．ARertwentv．fourhourS，thetmcheawascannulatedviaendotrachealintubation．Theratskeptspomaneousbreathing．TheVenacaudalisofratwascarmulatedwith24Gtrocar．SodiumlactatedRinger’ssolut

6、ionwasin几sed．ARerratwasplacedinastereotaxic印paratus，electroencephalo铲am(EEG)，electrocardiogram(ECG)andtherectaltemperaturewerecontinuouslymonitored．Rectaltemperaturewascontinuouslymaintainedat(37．O士O．5)℃bychangingahotwaterbagunderthetnmcusofaratandregulatingt11e5英文摘要hei

7、g『且tofa11incandescentl觚1pupthetnmcusofarat．Thebilateralcommoncarotidarterieswereblockedfor15minfollowedby8hreper如sion．weuseddi疏rentmetllodsaCcordingtodifferent铲oups．IngroupS，ratsreceivednles锄esu玛icalproceduresexceptt11atmebilateralVertebralarterieswerenotelectI．oca，uter

8、izedandmebilateralcommoncarotida眦erieswerenotoccluded．IngroupB，globalcerebralischemia-repermsionwasproduced，ra