资源描述：
《低温肠内喂养对胃缺血再灌注损伤大鼠影响的实验研究.pdf》由会员上传分享，免费在线阅读，更多相关内容在行业资料-天天文库。
1、护理研究2015年5月第29卷第5期上旬版(总第501期)·1575·低温肠内喂养对胃缺血再灌注损伤大鼠影响的实验研究戴利,罗利娜,曾秋霞摘要:[目的]探讨低温肠内喂养对胃缺血再灌注损伤(GIRI)大鼠影响及主要作用机制。[方法]将40只雄性SD大鼠随机分为肠内喂养温度10℃、24℃、32℃、40℃四组,采用夹闭腹腔动脉30min造成大鼠胃缺血,松开动脉夹后再灌注形成胃缺血再灌注损伤模型,手术后2h开始给予肠内营养(EN),术后48h观察胃黏膜损伤指数,采用免疫组织化学方法检测肿瘤坏死因子α(TNFα)在胃黏膜细胞中
2、的表达,采用ELISA法检测血清TNFα含量。[结果]10℃组肠内喂养胃黏膜损伤指数明显低于24℃组(P<0.05)、32℃组(P<0.01)、40℃组(P<0.01),胃黏膜TNFα阳性表达低于24℃组(P<0.05)、32℃组(P<0.05)、40℃组(P<0.01);胃黏膜损伤指数、TNFα阳性表达与肠内喂养温度均呈正相关(P<0.01);各组大鼠血清TNFα含量差异无统计学意义(P>0.05)。[结论]10℃肠内喂养温度对大鼠因缺血再灌注造成的急性胃黏膜损伤具有保护作用,其机制可能与低温抑制机体过度表达的炎症
3、反应有关。关键词:肠内喂养;温度;大鼠;胃缺血再灌注;急性胃损伤ExperimentalstudyoninfluenceoflowtemperatureenteralfeedingonratswithgastricischemiareperfusioninjuryDaiLi,LuoLina,ZengQiuxia(People’sHospitalofBao’anDistrictShenzhenCityGuangdongProvince,Guang-dong518101China)AbstractObjective:To
4、probeintotheinfluenceoflowtemperatureenteralfeedingonratswithgastricischemiareperfusioninjury(GIRI)anditsmainmechanisms.Methods:Atotalof40maleSpragueDawley(SD)ratswererandomlydividedintofourgroupsaccordingtoenteralfeedingtemperature:10℃,24℃,32℃and40℃(n=10pergro
5、up).Clampingtheceliacarteryfor30mintoleadgastricischemiaandthentoremovedthecliptomakeGIRImodel.At2hlaterafterreperfusion,allratsreceivedenteralfeeding.When48hlaterafterthereperfu-sion,gastricmucosalinjuryindexwascountedgrossly.Theexpressionoftumornecrosisfactor
6、α(TNFα)ofgastricmucosalcellwasdetectedbyimmunohistochemistry.AndtheplasmaTNFawasdetectedwithenzymelinkedimmunosorbentassay(ELISA).Results:ThegastricmucosalinjuryindexandpositiveexpressionofTNFαin10℃groupweresignificantlylowerthanthatintheothergroups(P<0.05orP<0
7、.01).Meanwhile,thosewerepositivelycorrelatedwithenteralfeedingtemperature(P<0.01).TherewasnostatisticallydifferenceintermsofplasmaTNFαcontentamongfourgroups(P>0.05).Conclusion:Theresultssuggestedthat10℃enteralfeedingshowagastroprotectiveeffectfortheischemiarepe
8、rfusioninjuryinrats,Themechanismmayberelatedtolowtemperatureinhibitingthebody’sinflammatoryresponseoverexpression.Keywordsenteralfeeding;temperature;rat;gastricischemiareper
