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时间:2019-02-27
《黄芪多糖对3t3-l1前脂肪细胞增殖和分化的影响》由会员上传分享,免费在线阅读,更多相关内容在工程资料-天天文库。
1、中西医结合学报2007年7月第5卷第4期JournalofChineseIntegrativeMedicine,July2007;Vol.5,No.4·421·黄芪多糖对3T3L1前脂肪细胞增殖和分化的影响刘毅,王文健,陈伟华,应健复旦大学中西医结合研究所,上海200040目的:探讨黄芪多糖(Astragaluspolysaccharides,APS)对前脂肪细胞增殖、分化的影响及其机制。方法:以XTT法检测3T3Ll前脂肪细胞的增殖;油红O染色并通过比色定量分析检测3T3L1前脂肪细胞分化过程中胞浆脂质的堆积;采用实时聚合酶链式反应和蛋白质免疫印迹(West
2、ernblotting)技术检测前脂肪细胞分化相关基因过氧化物体增殖剂活化受体γ(peroxisomeproliferatoractivatedreceptorγ,PPARγ)、CAAT/增强子结合蛋白α(CAAT/enhancerbindingproteinα,C/EBPα)mRNA以及蛋白质的表达。结果:APS浓度从0.025到0.8g/L,在24、48和72h各时间段对前脂肪细胞的生长均表现为增殖趋势,且呈一定的量效关系;0.4g/LAPS处理的前脂肪细胞,胞浆中出现较大量脂滴,其作用与噻唑烷二酮(thiazolidinedione,TZD)类药物罗格列酮(
3、rosiglitazone,ROS)相似;APS使前脂肪细胞分化相关基因PPARγ和C/EBPαmRNA与蛋白的表达明显增加,与空白对照组相比,差异有统计学意义(P<0.05,P<0.01)。结论:APS能够促进前脂肪细胞的增殖和分化,增加脂肪细胞分化过程中脂质的堆积,其机制可能与其促进PPARγ和C/EBPαmRNA与蛋白质表达有关。关键词:黄芪多糖;增殖;分化;mRNA;前脂肪细胞中图分类号:R285.5;文献标识码:A;文章编号:16721977(2007)04042106EffectsofAstragaluspolysaccharidesonproli
4、ferationanddifferentiationof3T3L1preadipocytesYiLIU,WenjianWANG,WeihuaCHEN,JianYINInstituteofChineseIntegrativeMedicine,FudanUniversity,Shanghai200040,ChinaObjective:ToobservetheeffectsofAstragaluspolysaccharides(APS)ontheproliferationanddifferentiationof3T3L1preadipocytesandtoeluc
5、idateitspossiblemechanism.Methods:Theproliferationof3T3L1preadipocyteswasdetectedbyXTTmethod.LipiddropletsaccumulatedincytoplasmofthedifferentiatedpreadipocyteswereobservedbyusingredOstainingandquantifiedbycolorimetry.Theexpressionsofperoxisomeproliferationactivatedreceptorγ(PPARγ)and
6、CAAT/enhancerbindingprotein(C/EBPα)mRNAsandproteinsweredetectedbyrealtimepolymerasechainreactionandWesternblottingrespectively.Results:APSatdifferentconcentrations(0.0250.8g/L)affected3T3L1preadipocyteproliferationanddifferentiationdosedependently.3T3L1preadipocytestreatedwith0.4g
7、/LAPShadlotsoflipiddropletsinthecytoplasma,whichweresimilartocellstreatedwithrosiglitazone(ROS).APSsignificantlyincreasedthemRNAandproteinexpressionsofPPARγandC/EBPα(P<0.05,P<0.01,comparedwiththenormalcontrolgroup)inthecourseof3T3LIpreadipocytedifferentiation.Conclusion:APScanpromot
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