体外冲击波对兔骨性关节炎软骨细胞凋亡与caspase-3活性影响的实验分析

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1、中文摘要骨保护功能。ESW是一种有前途的无创性治疗OA的方法，具有重要的临床意义和实践意义。关键词：体外冲击波；骨性关节炎；软骨细胞；细胞增殖；细胞凋亡；半胱天冬酶．3；一氧化氮英文摘要Effectofextracorporealshockwaveonchondrocyteapoptosisofrabbitosteoarthritismodelandactivityofcaspase··3ABSTRACTobjective：ExtracorporealShockWaveTherapy(ESWT)asoneoforiginalnonvirulencecurem

2、ethodinorthopedics，itexistsmanypredominanceinorthopaedicsurgeryandcuresoft．tissuediseasesthantraditionalsurgerymethods．Inrecentyears，ExtracorporealShockWave(ESW)hassignificancurativeeffectinosteoarthritis(OA)treatment．However，itsmechanismiSunclearlyinrelationmolecularreports．Additi

3、onal，scientistshavenotpaymuchattentiontotheroleofchondrocyteindevelopmentof0A．InordertodemonstrateESWasoneuninvasiontherapeuticsindelaydevelopmentof0Aandprovidetheoryfoundationforclinicalapplication，wedothisresearch．Thenormalchondroycytecultureinvitroisincentivedbydifferencelevelso

4、fenergyintensityandfrequencyofESW．ThepurposeistosievetheoptimizationenergyintensityandfrequencyofESWandtoguidetheESWuseinanimalexperiments．ThentoestablishOAmodelofrabbitkneewhichbeenexertedbyalreadyselectedESWanddetectapoptosisofchondrocyte．Besides，demonstrateifESWestistsmolecular5

5、英文摘要metabolismtopreventinflammation，apoptosisofchondrocyteandpromoterepairofcartilage．Methods：Primarypart：Afterattainnormalchondrocyteofrabbitkneebyenzymicmethod，thecellisincentivedbyvariouslevelsofenergyandfrequencyofESWSievetheopimizationESWparameterbyCCK．8andHEstain．Secondpart：e

6、stablismentOAmodelofrabbitkneebyACLT．ARerthesurgery,therabbitkneeofexperimentalgroupgiveESWtreatment。Rabbitsbeensacrificedattheperiodof4weeksand8weeks．Morphology,tissuestainandcaspase一3wereusedforstudy．DAPI，TUNELmethodandflowcytometryannexinVwereusedforthestudyofapoptosis．NOlevelsw

7、eremeasuredinrabbitsynovia．ExperimentaldataabovementionedanalysisbyANOVAandnonparameterranksumtest．APvalueof

8、rpnessandround，butthesizeh