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ID:33072722
大小:4.52 MB
页数:73页
时间:2019-02-19
《体外冲击波对兔骨性关节炎软骨细胞凋亡与caspase-3活性影响的实验分析》由会员上传分享,免费在线阅读,更多相关内容在行业资料-天天文库。
1、中文摘要骨保护功能。ESW是一种有前途的无创性治疗OA的方法,具有重要的临床意义和实践意义。关键词:体外冲击波;骨性关节炎;软骨细胞;细胞增殖;细胞凋亡;半胱天冬酶.3;一氧化氮英文摘要Effectofextracorporealshockwaveonchondrocyteapoptosisofrabbitosteoarthritismodelandactivityofcaspase··3ABSTRACTobjective:ExtracorporealShockWaveTherapy(ESWT)asoneoforiginalnonvirulencecurem
2、ethodinorthopedics,itexistsmanypredominanceinorthopaedicsurgeryandcuresoft.tissuediseasesthantraditionalsurgerymethods.Inrecentyears,ExtracorporealShockWave(ESW)hassignificancurativeeffectinosteoarthritis(OA)treatment.However,itsmechanismiSunclearlyinrelationmolecularreports.Additi
3、onal,scientistshavenotpaymuchattentiontotheroleofchondrocyteindevelopmentof0A.InordertodemonstrateESWasoneuninvasiontherapeuticsindelaydevelopmentof0Aandprovidetheoryfoundationforclinicalapplication,wedothisresearch.Thenormalchondroycytecultureinvitroisincentivedbydifferencelevelso
4、fenergyintensityandfrequencyofESW.ThepurposeistosievetheoptimizationenergyintensityandfrequencyofESWandtoguidetheESWuseinanimalexperiments.ThentoestablishOAmodelofrabbitkneewhichbeenexertedbyalreadyselectedESWanddetectapoptosisofchondrocyte.Besides,demonstrateifESWestistsmolecular5
5、英文摘要metabolismtopreventinflammation,apoptosisofchondrocyteandpromoterepairofcartilage.Methods:Primarypart:Afterattainnormalchondrocyteofrabbitkneebyenzymicmethod,thecellisincentivedbyvariouslevelsofenergyandfrequencyofESWSievetheopimizationESWparameterbyCCK.8andHEstain.Secondpart:e
6、stablismentOAmodelofrabbitkneebyACLT.ARerthesurgery,therabbitkneeofexperimentalgroupgiveESWtreatment。Rabbitsbeensacrificedattheperiodof4weeksand8weeks.Morphology,tissuestainandcaspase一3wereusedforstudy.DAPI,TUNELmethodandflowcytometryannexinVwereusedforthestudyofapoptosis.NOlevelsw
7、eremeasuredinrabbitsynovia.ExperimentaldataabovementionedanalysisbyANOVAandnonparameterranksumtest.APvalueof8、rpnessandround,butthesizeh
8、rpnessandround,butthesizeh
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