pge2通过pi3k2fakt信号转导通路激活creb促进胆管癌生长侵袭的研究

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1、南京医科大学硕士学位论文ofHuCCT1cellsandtheprobablesignaltransductionpmhway．Objectives：ToinvestigmetheeffectofPGE2ontheactivationofCREBanditsrelatedmechanisminHUCCT1cells．Methods：1．41casesofintrahepaticcholangiocarcinomatissueand20casesofnormalbileducttissuefromtheFirstAffiliatedHospitalofNanjingMedical

2、UniversitywerecollectedtodetecttheexpressionofPhospho-CREBbyimmunohistochemistry．TherelationshipbetweentheexpressionofPhospho—CREBandclinicopathologicalparametersWasanalyzedstatistically．2．ThecholangiocarcinomaHuCCT1cellswereculturedinconventionalapproach．3．HuCCT1cellsweretreated、Ⅳitllexogen

3、ousPGE2，cellsmigrationandinvasionWasexaminedbyTranswell．4．HuCCTlcellsweretreatedwithexogenousPGE2for0min、5min、15min、30min、45min、60minrespectively,WesternblottestwasconductedtoobservethealterationofPhospho—CREB．5．HuCCT1cellsweretreatedwithexogenousPGE2，theconcentrationofCa2+inthecellwasexamin

4、edbylaserscanningconfocalmicroscope．6．WesternblottestwasperformedtoinvestigatetheexpressionlevelofPhospho-CREBinHuCCT1cellsafterthetreatmentofPGE2，PGE2+BAPTA-AM．7．WesternblottestwasperformedtoinvestigatetheexpressionlevelofPhospho—CREBinHuCCT1cellsafterthetreatmentofPGE2，PGE2+LY294002．南京医科大学

5、硕士学位论文Results：1．ImmunohistochemistrywasusedtodetecttheexpressionofPhospho-CREBin41casesofintrahepaticcholangiocarcinomatissueand20casesofnormalbileducttissue．ThepositiveexpressionratesofPhospho-CREBin41casesofintrahepaticcholangiocarcinomaand20casesofnormalbileducttissuewere73．2％and35．O％．res

6、pectively(尸<0．05)．TheexpressionofPhospho-CREBwasnotstatisticallyrelatedtosex，age，tumorsize，TNMstaging，lymphnodemetastasisandperineuralinvasion．ThepositiveexpressionratesofPhospho—CREBinhigh-moderatelydifferentiatedtumorsandpoorlydifferentiatedtumorswere59．1％and89．5％．respectively(P

7、llinvasionwasfoundtoincreaseby3．2timeswhenthecellsweretreatedwith59MPGE2for24h．3．HuCCT1cellsweretreatedwith5p,mol／LPGE2for5min、15min，30min、45min、60min，theWestemblottestshowedthatthelevelofPhospho-CREBwasincreasedby64．5％、135．5％、141．9％、98．4％、59．7％，re