her2基因对恶性脑膜瘤iomm-lee细胞株增殖和侵袭的影响

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1、摘要结论：1、HER2可促进恶性脑膜瘤细胞的增殖和侵袭；2、HER2基因可能通过MAPK信号通路影响恶性脑膜瘤细胞的生长、侵袭功能。关键词：恶性脑膜瘤；HER2；MAPK；增殖；侵袭IIIAbstractABSTRACTObjective：ToinvestigatetheinfluenceofHER2geneontheproliferationandinvasionofIOMM-Leemaligantmeningiomacellline,andtodiscusswhethertheHER2genemakethetheproliferationandinva

2、sivenesseffectsbyMAPKsignaltransductionpathway.Methods:1、IOMM-LeemaligantmeningiomacelllineswereinfectedbysilencingandoverexpressionofHER2geneHER2plamidwithlentivirusvectors.Theexperimentwasdividedintofivegroups:thefirstgroupwasuntreated(blank),thesecondgroupwasnegativecontrolofsi

3、lence(NC-sh),thethirdgroupwasthesilenceofHER2gene(HER2-sh),thefourthgroupwasnegativecontrolofoverexpressing(NC-ox),thefifthgroupwastheoverexpressingofHER2gene(HER2-ox).TheexpressionofHER-2genewasevaluatedbyq-PCRandWesternblot.2、TheMTTexperiment,andEdULabelingassayweretoinvestgatet

4、heproliferationabilityofdifferentgroups.CellinvasionandmigrationanalysiswereinvestgatedbyMatrigelinvasionassayandWoundhealingassay.Cellcycleandapoptosisassaysbyflowcytometryweretoinvestgatethecellcyclesandtheabilityofapotosis.3、Westernblot.wereusedtodetecttheexpressionofERK1/2，P-E

5、RK1/2，JNK，P-JNK，ERK5、P-ERK5、P38、P38andrasprotein.4、Thenudemicehasbeeninocµlatedwithcellsfromeachgroupsthoughtheirarmpits,then,observethetimeofformingandtheweighoftumorsinthenudemice.IVAbstractRsµlts:1、theplasmidofsilencingandtheoverexpressiontheHER2wereestablishedsuccessfµlly.Thel

6、evelofmRNAandproteinofHER2reachedtherequirementsofeachgroups.2、Theproliferation,invasionandmigrationofthemeningiomacellswithadown-regµlationofHER-2byshRNAhumanlyweresignificantlyreduced.Whiletheproliferation,invasionandmigrationofthehumanmeningiomacellswithaup-regµlationofHER-2bys

7、hRNAweresignificantlyenhanced.TheinfluenceofHER2geneinthecellcyclewasassociatedwithG0/G1-phasetoS-phase.TheapoptosisofsilencingHER2groupwasincreasedandtheapoptosisofoverexpressionHER2groupwasdecreased.3、Theinfluenceofdown-regµlationandup-regµlationtheHER2genetakeeffectsonERK1/2、P-

8、ERK1/2、JNK、P-JNK、ERK5、P-ERK5、rasi