嗜酸乳杆菌的分离、鉴定与其在发酵豆乳中生长特性的.研究

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1、InChina，soymilkisatraditionalfood，butalsoakindofcheapandeasyaccessibleresource．Butduetotheimpactofthetraditionalhabits，peoplehavebeenunabletorecognizethebenefitsofsoymilk．addition，soymilkhasheavierbeanyflavor,SOthatmanyconsumers，especiallyteenagersandchildrenhavelowlevelofa

2、cceptance·Therefore，theuseofmodembiologicaltechnique，itisofgreatsignificancetodevelopfunctionalfermentedsoymilkbeveragesthroughthefermentationoflacticacidbacteria．Thesebeveragescannotonlysolvetheproblemoftaste，butalsoprovidehighqualityandinexpensivefunctionalvegetableprotei

3、nbeveragefortheregionswhichareshortofmilk．thisstudy,Lactobacillusstrainswereisolatedfromfreshinfantfeces．TherewerethreeLactobacillusacidophilusstrainsthroughmolecularlybiologicalidentification．ThisstudyisaimtoprovideLactobacillusacidophilusstrainswithstronggrowthcapacity，hi

4、ghactivenumberandstrongacid—producingabilityforfermentedsoymilk．Besides，weexploredthegrowthcharacteristicsofLactobacillusacidophilusinfermentedsoymilk，providingatheoreticalbasisandascientificbasisforindustrializedproductionoffermentedsoymilk．Bycomparingthebiologicalcharacte

5、risticsofthethreestrains，includingthegrowthcurve，acid—producingability，gastricIV浙江工商大学硕士学位论文acid·-resistantcapacity,bile-·resistantcapacity,antibioticresistanceandantibacterialcapacity，wechoosedLactobacillusacidophilusLA一5astestedstrain．TheactivenumberofLactobacillusacidoph

6、ilusLA一5canreachto4．33X1012cfu／mLaftercultivatedinMRSliquidmediumfor14hours．LactobacillusacidophilusLA一5hasstrongacid—resistantandbile—resistantcapacity．Thestrainishighlysensitivetochloramphenicol，penicillinG，rifampicinandacheomycin，butis’tsensitivetokanamycin．Invitro，Theme

7、tabolitesofLactobacillusacidophilusLA一5areobviousantagonismonE．coli，SalmonellaandStaphylococcusaureusandStaphylococcuscitreus．InordertodevelopaLactobacillusacidophilusstartersuitableforproducingfermentedsoymilk，westudiedonthegrowthpromotingcultureofLactobacillusacidophilusL

8、A一5basedonsoymilk．Theoptimalgrowthpromotingculturecompositionwasasfollowing：10％soy