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1、ABSTRACTABSTRACTThehumanbodyiscolonizedbyacomplexcommunityofmicrobeswithdifferentfunctionstermedmicrobiotaormicrobiome,anditiscriticalforhumanhealth.ProbioticsuchasLactobacillusacidophilussupplementbeneficiallyaffectsthehostbyimprovingitsintestinalmicrobialbalance.Ithas
2、beenimplicateddistincthost-derivedLactobacillusacidophilusexhibitdifferentadhesionpropertiesspecificallytohostafterprolongedofevolution.Thuswepayurgentattentiontothescreeningofhuman-derivedLactobacillusacidophiluswhichkeepalivethroughthegastrointestinalchannel,bacterios
3、tasisactiveness,andexcellentadhesionpropertiestoviscerallumen.Andgoafterwardsforfunctionalfoods,forinstance,activemicrobialagentsorincludeinhealthcareproducts.TooptimizetheprocessofLactobacillusacidophiluscultureplaysanextremelyimportantroleinitshighcelldensityculture(H
4、CDC),evenmoreimportant,preparationofmicrobialagentsusingvacuumfreeze-dryingtoprotectbioactivity,andthesemanipulationsarethecoretechnologytoincreaseproductivity,ensureproductstandardization,maintainproductstability,extendshelflife.UselowpH/highconcentrationsofbilesaltsme
5、diumforprimarilyscreenofLactobacillusacidophilusfrom7individual7to14-dayneonatalfaeces,and8gram-positivebacteriumwhichexhibitacidtoleranceandbilesaltresidencewasobtained.2wereselectedafteridentificatedbycolonymorphology,physiologicalandbiochemicalcharacteristicsandmolec
6、ularidentification,werenamedLactobacillusacidophilusLaNCU401andLaNCU402,respectively.ThestudyofLaNCU402invitroshowed:LaNCU402exhibithightoleranceinartificialgastrointestinalfluidwhenafter12-hourmanipulation,theviablecellconcentrationremainsmorethan107cfu/mL;preferablyad
7、hesionpropertiestoCaco-2cellswhileadhesionindexattained9.52±1.40/Caco-2;goodinhibitionofpathogenicbacteriabuthadnoinfluenceonlacticacidbacteria;andhadaresembledrugresistancetoLactobacillusacidophilusATCC4356.IVABSTRACTConfirmtheculturemediumspecificallyforLaNCU402byusin
8、gTwo-levelFractionalFactorialDesign,SteepestAscent,CentralCompositeDesign,Optimizedformula:glucose1.5%,peptone
