核糖体展示人单链抗体库的构建与抗hcv+ns5b蛋白的scfv的筛选

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1、-------第四军医大学硕士学位论文独创性明声秉承学校严谨的学风与优良的科学道德，本人声明所呈交的论文是我个人在导师指导下进行的研究工作及取得的研究成果。尽我所知，除了文中特别加以标注和致谢的地方外，论文中不包含其他人已经发表或撰写过的研究成果，不包含本人或他人已申请学位或其他用途使用过的成果。与我一同工作的同志对本研究所做的任何贡献均已在论文中作了明确的说明并表示了致谢。申请学位论文与资料若有不实之处，本人承担一切相关责任。论文作者签名：日期：保知明护识产权声本人完全了解第四军医大学有关保护知识产权的规定，即：研究生在校攻读学位期间论文工作的知识产权单位属

2、第四军医大学。本人保证毕业离校后，发表论文或使用论文工作成果时署名单位仍然为第四军医大学。学校可以公布论文的全部或部分内容（含电子版，保密内容除外），可以采用影印，缩印或其他复制手段保存论文。学校有权允许论文被查阅和借阅，并在校园网上提供论文内容的浏览和下载服务。同意学校将论文加入《中国优秀博硕士学位论文全文数据库》和编入《中国知识资源总库》，同意按《中国优秀博硕士学位论文全文数据库出版章程》规定享受相关权益。论文作者签名：导师签名：日期：------------1------------第四军医大学硕士学位论文核糖体展示人单链抗体库的构建及抗HCVNS5B蛋

3、白的ScFv的筛选研究生：田江红学科专业：微生物学所在单位：第四军医大学微生物学教研室导师：徐志凯教授辅导教师：尹文教授雷迎峰讲师资金资助项目：国家自然科学基金关键词：HCV；NS5B蛋白；单链抗体库；核糖体展示技术中国人民解放军第四军医大学2010年5月-0------------第四军医大学硕士学位论文目录英文缩略语表····································································错误！未定义书签。中文摘要········································

4、······································································4英文摘要··············································································································8前言·······························································································

5、·············13文献回顾············································································································14正文············································································································30第一部分截短型HCVNS5B基因克隆、表达及纯化··················

6、···············301材料·········································································································302方法··········································································································343结果-----------·······························

7、··········································································414讨论······································································4错误！未定义书签。第二部分核糖体展示人单链抗体的构建及筛选针对HCVNS5B的ScFv与表达……………………………………………………………………………481材料·····················································

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