神经降压素对帕金森病模型大鼠纹状体da含量影响论文

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1、神经降压素对帕金森病模型大鼠纹状体DA含量影响论文【摘要】目的观察神经降压素(NT)对正常和帕金森病模型大鼠纹状体多巴胺(DA)及其代谢产物含量的影响。方法采用6羟基多巴胺(6OHDA)单侧损毁内侧前脑束制备帕金森病大鼠模型。将5μL1mmol/LNT或生理盐水双侧缓慢注入正常及帕金森病模型大鼠侧脑室30min后，利用高效液相色谱分析(HPLC)法检测纹状体DA及其代谢产物高香草酸(HVA)和3,4二羟基苯乙酸(DOPAC)含量的变化。结果正常大鼠NT组纹状体DA含量与生理盐水组相比均明显升高(t=5.32，P0.01)。帕金森病模型大鼠生理盐水组损毁侧纹状体DA含量与未损毁

2、侧和正常大鼠生理盐水组相比明显降低(F=61.23，P0.001);损毁侧纹状体DA更新率(DOPAC+HVA)/DA、DOPAC/DA、HVA/DA均明显高于未损毁侧和正常大鼠生理盐水组(F=27.52、21.12、28.43，P0.001)。帕金森病模型大鼠侧脑室注射NT可明显提高两侧纹状体DA的含量(t=3.87、2.26，P0.05)。结论NT可增加正常及帕金森病模型大鼠纹状体DA的含量，提示NT具有抗帕金森病效应。【关键词】神经降压素;帕金森病;纹状体;多巴胺;色谱法,液相;大鼠ABSTRACTObjectiveToobservetheeffectsofneurotens

3、inonstriataldopaminecontentinnormalandParkinsonianrats.MethodsAratmodelofParkinsondisease(PD)createdbyunilateraladministrationof6hydroxydopamine(6OHDA)intothemedialforebrainbundle.Afterbilateralintracerebroventricularadministrationof1mmol/Lneurotensinorsaline(5μL),highperformanceliquidchroma

4、tography(HPLC)ineanditsmetabolites:homovanillic(HVA)and3,4dihydroxyphenylaceticacid(DOPAC).ResultsInnormalrats,microinjectionofneurotensinsignificantlyelevatedtheconcentrationofdopamineinthestriatum(t=5.32,P0.01).InPDmodelrats,thestriataldopaminelevelonthelesionedsidealrats(F=61.23,P0.01),icr

5、oinjectionofneurotensinsignificantlyelevatedthestriataldopaminelevelonbothsidesofPDmodelrats(t=3.87,.freelinelevelinstriatumonbothnormalandPDmodelrats,ayexertantiparkinsonianeffects.KEYJ,etal.NeurotensinanddopamineinteractionsJ.PharmacolRev,2001,53(4):453486.2BOULESM,L,JENNERP,etal.Neurotensi

6、n,substanceP,deltaandmuopioidreceptorsaredecreasedinbasalgangliaofParkinson’sdiseasepatientsJ.Neurosci,1994,61(1):7379.6CHINAGLIAG,PROBSTA,PALACIOSJM.NeurotensinreceptorsinParkinson’sdiseaseandprogressivesupranuclearpalsy:anautoradiographicstudyinbasalgangliaJ.Neurosci,2003,39(2):351360.7MAR

7、TORANAA,FUSCOFR,D’ANGELOV,etal.Enkephalin,neurotensin,andsubstancePimmunoreactiviteneuronesoftheratGPfolloinelesionofthesubstantianigraJ.ExpNeurol,2003,183(2):311319.8魏磊,陈蕾.苍白球注射5HT对氟哌啶醇所致大鼠僵直症状影响J.青岛大学医学院学报,2008,45(4):316318.9张淑静,陈蕾