司来吉兰对帕金森病模型大鼠黑质纹状体TH及GDNF表达的影响.pdf

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1、154TianjinMedJ，February2015，Vo1．43No．2司来吉兰对帕金森病模型大鼠黑质纹状体TH及GDNF表达的影响吕超男，毛文静，马原源，刘斌，张晋霞，孙静，成晓华，李世英摘要：目的观察司来吉兰对帕金森病(PD)模型大鼠黑质纹状体内酪氨酸羟化酶(TH)及胶质细胞源性神经营养因子(GDNF)表达的影响，探讨司来吉兰对多巴胺能神经元的保护作用及机制。方法72只健康雄性sD大鼠随机分为对照组、模型组和司来吉兰组，每组均设4d和8d2个亚组，各l2只。模型组和司来吉兰组采用颈背部皮下注射鱼藤酮制备PD模型，对照组

2、皮下注射等体积葵花油。之后司来吉兰组每日灌胃咪多吡0．5mg／kg，模型组和对照组每13灌胃等体积生理盐水，4d和8d组分别连续灌胃4d和8d。采用免疫组化法和Westernblotting法检测黑质纹状体TH和GDNF表达水平。结果免疫组化法和Westernblotting法检测结果均显示，对照组大鼠黑质纹状体可见多量TH阳性细胞表达和少量GDNF阳性细胞表达，8d和4d组差异均无统计学意义。模型组TH和GDNF阳性细胞表达均明显低于对照组(均P<0．05)，8d和4d组差异均元统计学意义。司来吉兰组TH阳性细胞表达低于对照

3、组而高于模型组，GDNF阳性细胞表达高于对照组和模型组(均P

4、ntianigraandstriatuminParkinson’sdiseasemodeIinratLYUChaonan，MAOWenjing，MAYuanyuan，LIUBin，ZHANGJinxia，SUNJing，CHENGXiaohua，LIShiyingFirstDepartmentofNeurology,theAffiliatedHospitalofHebeiUnitedUniversity,Tangshan063000,ChinaCorrespondingAuthorE—mail：liubintsh@126．co

5、mAbstract：0bjectiveToobservetheeffectsofEldeprylonexpressionsoftyrosinehydroxylase(TH)andglialcellline—derivedneurotrophicfactor(GDNF)insubstantianigraandstriatuminParkinson’sdisease(PD)andtoexploretheprotectivemechanismofEldeprylondopaminergicneuron．MethodsHealthym

6、aleSprague—Dawley(SD)rats(n=72)wererandomlydividedintocontrolgroup，modelgroupandEldeprylgroup(n=24ineachgroup)．Eachgroupwasdividedrandom—lyinto2subgroupsas4daytreatmentgroupand8daytreatmentgroup(n=12ineachsubgrop)．Pakinson’sdiseasemodelwasestablishedbyinjectingroten

7、onesubcutaneouslybacktheneck，ratsinthecontrolgroupwerei~ectedwithanequalvol—umeofsunfloweroilsubcutaneouslyatthesamelocation．RatsintheEldeprylgroupwerethengivenEldepryl0．5mg·kg—in—tragastrieallyeverydayfor4or8consecutivedaysandratsinmodelgroupandcontrolgroupweregive

8、nanequalvolumeofsalineinstead．TheexpressionofTHandGDNFinsubstantianigraandstriatumweredetectedbyimmunohistochemistryandWesternblotting．Res