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1、广东药学院硕士研究生学位论文重现性好，测得复合物滴丸含量为8.11±0.11%。通过体外溶出度实验证明，相比于复合物，复合物滴丸体外溶出得到显著改善。3.SA滴丸与复合物滴丸在大鼠体内药动学研究建立了HPLC法测定大鼠血浆中SA浓度的方法，结果SA线性范围为：0.8～10μg·mL-1，定量下限为0.8μg·mL-1，检测限0.3μg·mL-1，SA提取回收率大于85%，准确度与精密度符合要求，专属性强，血浆样品稳定性好。分别给SD大鼠灌胃SA滴丸和SA磷脂复合物滴丸，给药剂量（以SA含量计）为500mg·Kg-1。得到其Tm
2、ax分别为33.54min和53.22min，Cmax分别为2.099μg·mL-1和4.248μg·mL-1，AUC分别为7.576μg·h·mL-1和18.562μg·h·mL-1。相比于SA滴丸，SA复合物滴丸AUC增加2.45倍，半衰期延长，消除速率常数减小，说明SA复合物滴丸比SA滴丸吸收更好，体内作用时间延长，提高了SA在大鼠体内的生物利用度。关键词：SA；磷脂复合物；滴丸；药动学II万方数据广东药学院硕士研究生学位论文ThepreparationofSAphospholipidcomplexdrippingpil
3、lsandthestudyonitspharmacokineticsinratsYanyanYang(TraditionalChineseMedicine)Supervisor:Prof.ChongkaiGaoABSTRACTObjective:SAisamajorwater-solublecomponentsofSalviamiltiorrhiza,withawiderangeofpharmacologicaleffectsandclinicalapplications,especiallyforthetreatmentof
4、cardiovasculardiseases.SAhasgoodwatersolubility,butpoororalabsorptionandbioavailability.Inthispaper,SAphospholipidcomplexwasprepared,inordertoimprovetheoralabsorptionandbioavailability.Finally,wepreparedtheSAphospholipidcomplexdrippingpills.Itisconvenientforpatients
5、totake,whichlaidafoundationforitsindustrialization.MethodsandResults:3.PreparationofSAphospholipidcomplexSAphospholipidscomplexwaspreparedbysolventevaporationmethod.ThebindingrateofSAwasinvestigated,singlefactortestinvestigationwereappliedtoanalyzethetechnologicalpa
6、rameters,suchasthereactionsolvent,themassratioofSAandlipid,theconcentrationofSA,reactiontemperatureandtime.Theoptimumprocessparameterswereasfollows:usingethanolasreactionsolvent,reagentsproportionofSAandphospholipidas1:1,themassconcentrationofreactionsolutionofSAwas
7、10mg•mL-1,reactingfor1hat40℃.Inaddition,theexperimentestablishedthemethodforthedeterminationofSAphospholipidcomplex.Theresultsshowthat,themethodisspecific,accuratelyandsensitivity.SAwaslinearovertheconcentrationrangeof1~50µg•mL-1.Theregressionequationasfollows:A=188
8、46C-16857,r=0.9997.ThecontentofSAphospholipidcomplexIII万方数据广东药学院硕士研究生学位论文was34.59±0.46%,whichwasdeterminedbythismethod.Finally,NMRandDTAwa
