丹参醇提物在大鼠体内的药动学研究

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1、.1480·中草药ChineseTraditionalandHerbalDrug，第36卷第10期2005年10月丹参醇提物在大鼠体内的药动学研究李莉，蒋学华，衷媛，王凌，杨俊毅，周静(四川大学华西药学院.四川成都610041)摘要;月的建立大鼠血浆中丹参酮I;和隐丹参酮的HPLC-UV检测法，并研究丹参脂溶性成分在大鼠体内的药动学。方法以吉非罗齐为内标，血浆样品经简单的甲醇沉淀蛋白后.上清液直接进样测定大鼠尾Iv丹参醉提取物(以丹参酮2*计为5mg/只少，侧定各时点丹参酮叭和隐丹参酮的血药浓度绘制药时曲线，采用3p87

2、计算药动学参数。结果大鼠尾;v丹参醇提取物后，丹参酮I，的主要药动学参数分别为i,:为((0.088士。.024)h.t,。为(2.1士0.7)h:V为(0.8士0.6)L;CL为(2.0士1.1)L/h’;AUC}，和AUC,_分别为(2.2士0.9)mg·h/L和((3.4士1.7)mg"h/I结论丹参醇提取物iv给药后，丹参酮I，的药一时曲线符合二室模型血浆中丹参酮I*迅速下降，表观分布容积大;隐丹参酮在血浆中消除快，药动学参数不易获得关键词丹参;丹参醇提取物;丹参酮皿。;隐丹参酮药动学;高效液相色谱中图分类号82

3、86.02;8285.01文献标识码:13文章编号0253一2670(2005)10一1480一03PharmacokineticsofalcoholextractofRadixSalviaeMiltiorrhiazeinratinvivaI_ILi,JIANGXue-hua.YUANYuan,WANGLing,YANGJun-yi,ZHOUJing(WestChinaSchoolofPharmacy.SichuanUniversity,Chengdu610041,China)Abstract:ObjectiveToes

4、tablishasensitiveHPLCmethodwithUVdetectionforthedeterminationoftanshinoneff、(’I'SHA)andcryptotanshinone(CT)inratplasmaandtostudypharmacokineticsofRadixSalviaeMiltiorrhizae(RSM)liposolublecomponentsinratinvivo.MethodsTS9}andCTinplasmaofratatdifferentsamplingtimewe

5、redeterminedbypretreatmentwithGemifibrozilasinternalstandard,theproteinwasprecipitatedinmethanolsolutionafterivadministrationofalcoholextractofRSM5mg/peranimal.Themeanplasmaconcentration-timecurvewasprotractedandpharmacokineticparameterswerecalculatedby3p87.Resul

6、tsThemainpharmacokineticparametersofTSII,,wereasfollows:t112,was(0.088士0.024)h;t=ypwas(2.1士0.7)h;Vcwas(0.8士。6)L;CLwas(2.0士1.1)1.小一’;AUCo,andAUC_were(2.2士0.9)mg·h/1.and(3,4士1.7)mg·h/I，respectivelyConclusionTheconcentration-timecurveofTS1,Acanbefittedtotwo-compartm

7、entmodelafterethanolextractofRSMivadministrated.ConcentrationofTSDainratplasmadeclinesrapidlywithlargeapparentvolumeofdistribution.CTdecreasesalsorapidlyanditisdifficulttoacquireitspharmacokineticparametersinrat.Keywords:RadixSalviaMiltiorrhiza(RSM);alcoholextrac

8、tofSalviaMiltiorrhizaRadix(AERSM)tashinoneIIA(TSII*);cryptotanshinone(CT);pharmacokinetics;HPLC丹参脂溶性成分系从唇形科鼠尾草属植物丹参病、心脑血管疾病，效果满意37。本实验建立了血浆SalviamiltforrhizaBu