ang ii受体阻断剂对系膜增殖型肾炎家兔系膜基质影响

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1、AngII受体阻断剂对系膜增殖型肾炎家兔系膜基质影响【摘要】目的探讨血管紧张素II受体阻断剂洛沙坦对系膜增殖型肾炎家兔系膜细胞增殖、系膜基质增多的的药效和作用特点。方法将雄性日本大耳白兔30只随机分成正常对照组、病理模型对照组和洛沙坦治疗组。建立家兔系膜增殖型肾炎模型。双缩脲法测定洛沙坦对系膜增殖型肾炎家兔24h尿蛋白排泄量；放免法测定血浆中内皮素及降钙基因相关肽的水平；自动图像分析仪测定各组肾小球的定量指数，系膜基质指数；用免疫组化法观察肾组织增殖性细胞核抗原(PCNA)表达。结果与病理模型对照组相比，洛沙坦治疗组家兔24h尿蛋白排泄量明显下降(

2、P<0．05)，系膜面积显著减少(P<0．05)，内皮素(ET-1)降低、降钙素基因相关肽(CGRP)升高(P<0．05)，PCNA阳性细胞数减少(P<0．05)。结论洛沙坦具有调节内皮素与降钙素基因相关肽病理性改变，减少系膜基质的增多，及系膜细胞的增殖，从而对肾炎家兔有明显的保护作用。�【关键词】洛沙坦;系膜增殖型肾小球肾炎;内皮素;降钙素基因相关肽;系膜基质Effectoflonsartanonmesangialmatrixofmesangialproliferativeglomerulonephritisrabbits9SUNShi-jun，

3、DUXiao-yang,LIGuang-yuan.People’sHospitalofZhongshanCity，GuangdongProvince，528400，China�【Abstract】ObjectiveToinvestigatetheeffectofangiotensinIIreceptorantagonist,lonsartanontheclinichistologicalchangeofMSPGNrabbits.Methods30malerabbitswererandomlydividedinto3groups:Thenormalc

4、ontrolgroups(Ngroups).Theuntreatedgroups(Mgroup).Thelonsartantreatedgroups(Lgroup).Therabbitsmesangialproliferativeglomerulonephritis(MSPGN)modelswereestablished.24hours’excretionquantityofurineprotein(EQUP)wasdeterminedeveryweek,thelevelsofplasmaendothelin-1(ET-1)andCalcitoni

5、ngene-relatedpeptide(CGRP)weretestedwithradioimmunoassay(RIA)mesangialMatrixexpansionwereevaluatedwithquantitativepathologicanalysis.immunohistochemicalstainforproliferatingcellnuclearantigen(PCNA)wasperformedaftertreatment.ResultsEQURmesangialmatrixexpansionET-1inLgroupweresi

6、gnificantly9lowerthanthoseinMgroup(P<0．05)WhileCGRPwasremarkablyhigher(P<0．05).ThenumberofPCNAwaslessremarkably(P<0．05).DamageofrenaltissueinLgroupwasslightercomparedwiththatinMgroup.ConclusionCaptoprilcandecreasemesangialmatrixexpansion,alleviatetheinjuryofrenaltissueandobtai

7、nmarkedlytherapeuticeffect.�【Keywords】Lonsartan;Mesangialproliferativeglomerulonephritis;Endothelin-1;Calcitoningene-relatedpeptide;Mesangialmatrix血管紧张素II受体阻断剂(angiotensinIIreceptorantagonist,，ARB)可延缓肾功能不全的进展并减轻肾脏损伤，它不仅通过改善肾脏血流动力学，还通过改善肾小球的增殖而保护肾脏。关于内皮素-1(endothelin-1，ET-1)与降钙

8、素基因相关肽(calcitoningene-relatedpeptide，CGRP)介导的肾小球内增殖机制目前研究的很多。