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1、J.gen.Virol.(1987),68,1883-1890.PrintedinGreatBritain1883Keywords:TGEV/peplomerproteinE2/genesequenceThePredictedPrimaryStructureofthePeplomerProteinE2ofthePorcineCoronavirusTransmissibleGastroenteritisVirusByDENISRASSCHAERTANDHUBERTLAUDE*lnstitutNationaldelaRechercheAgronomique,Stati
2、ondeRecherchesdeVirologieetd'Immunologie,F-78850Thiverval-Grignon,France(Accepted31March1987)SUMMARYThecompletenucleotidesequenceofclonedcDNAscontainingtheE2glycoprotein-encodingregionofthegenomeoftransmissiblegastroenteritisvirus(TGEV)hasbeendetermined.Asinglelargetranslatableframeof
3、4.3kbstartingat8.2kbfromthe3'endofthegenomewasidentified.Itsdeducedaminoacidsequencecontainsthecharacteristicfeaturesofacoronaviruspeplomerprotein:(i)theprecursorpolypeptideofTGEVE2is1447residueslong(i.e.285longerthantheavianinfectiousbronchitiscoronavirusspikeprotein);(ii)partialN-te
4、rminalsequencingdemonstratedthataputativesecretorysignalsequenceof16aminoacidsisabsentinthevirion-associatedprotein;(iii)thepredictedmol.wt.oftheapoproteinis158K;mostofthe32potentialN-glycosylationsitesavailableinthesequencearepresumedtobefunctionaltoaccountforthedifferencebetweenthis
5、andtheexperimentallydeterminedvalue(200Kto220K);(iv)atypicalhydrophobicsequenceneartheCterminusislikelytoberesponsibleforanchoringthepeplomertothevirionenvelope.INTRODUCTIONTransmissiblegastroenteritisvirus(TGEV),ahighlyenteropathogenicvirusofpigs,belongstothefamilyCoronaviridae,agrou
6、pofenvelopedviruseswithalarge,positive-strandedRNAgenome(seeSiddelletal.,1983).StudiesontheorganizationandexpressionoftheTGEVgenome(Dennis&Brian,1982;Huetal.,1984;Jacobsetal.,1986;Kapke&Brian,1986;Rasschaertetal.,1987)tendtoconfirmthefindingsreportedfortwoothermembersoftheCoronavirida
7、e,murinehepatitis(MH)andavianinfectiousbronchitis(IB)viruses(seeLaudeetal.,1987).ThreefunctionalclassesofpolypeptideshavebeenidentifiedinTGEVvirions:anucleocapsidprotein,amatrixproteinandapeplomerproteinformingthecharacteristicsurfaceprojections(Garwesetal.,1976).ThepeplomerproteinE2,
8、ahigh
