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1、VirusResearch,10(1988)II-9477ElsevierVRR00405AntigenicstructureoftheE2glycoproteinfromtransmissiblegastroenteritiscoronavirusIsabelCorrea,Gustav0Jimhez,CarlosSuiiC,MariaJ.BullidoandLuisEnjuanesCentrodeBiologiaMolecular(CSIC-UAM),Fact&addeCiencias,UniversidadAutbnoma,CantoBlanco,28049M
2、adridSpain(Acceptedforpublication30December1987)Theantigenicstructureoftransmissiblegastroenteritis(TGE)virusE2glycopro-teinhasbeendefinedatthreelevels:antigenicsites,antigenicsubsitesandepitopes.Fourantigenicsites(A,B,CandD)weredefinedbycompetitiveradioimmunoassay(RIA)usingmonoclonal
3、antibodies(MAbs)selectedfrom9fusions.About20%(197)ofthehybridomasspecificforTGEvirusproducedneutralizingMAbsspecificforsiteA,whichwasoneoftheantigenicallydominantdeterminants.SiteAwasdifferentiatedinthreeantigenicsubsites:a,bandc,bycharacterizationof11MAbresistant(mar)mutants,thatwere
4、definedby8,3,and3MAbs,respectively.Thesesubsiteswerefurthersubdividedinepitopes.Atotalof11epitopesweredefinedinE2glycoprotein,eightofwhichwerecriticalforvirusneutralization.NeutralizingMAbswereobtainedonlywhennativeviruswasusedtoimmunizemice,althoughtoproducehybridomasmiceimmunization
5、sweremadewithantigeninthenative,denatured,ormixturesofnativeanddenaturedform.AllneutralizingMAbsreactedtoconformationalepitopes.TheantigenicstructureoftheEZglycoproteinhasbeendefinedwithmurineMAbs,buttheantigenicsiteswererelevantintheswine,thenaturalhostofthevirus,becauseporcineserare
6、actedagainstthesesites.MAbsspecificforTGEvirussiteCreactedtonon-immuneporcinesera.Thisreactivitywasnotdirectedagainstporcineimmunoglobulins.TheseresultsindicatedthatTGEviruscontainsepitope(s)alsopresentinsomenon-immunoglobulincompo-nentofporcineserum.TGEvirus;AntigenicstructureCorresp
7、ondenceto:L.Enjuanes,CentrodeBiologiaMolecular(CSIC-UAM),FacultaddeCiencias,UniversidadAuGnoma,CantoBlanco,28049Madrid,Spain.0168-1702/88/$03.5001988ElsevierSciencePublishersB.V.(BiomedicalDivision)78IntroductionTransmissiblegastroenteritisvirusofswineisamemberoftheCoronauiriduefamily
8、thatc