冠状病毒Coronavirusl论文-2013 Antiviral effects of a probioticEnterococcus faeciumstrain against transmissible gastroenteritis coronavirus.pdf

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1、ArchVirol(2013)158:799807DOI10.1007/s00705-012-1543-0ORIGINALARTICLEAntiviraleffectsofaprobioticEnterococcusfaeciumstrainagainsttransmissiblegastroenteritiscoronavirusWeidongChai•MichaelBurwinkel•ZhenyaWang•ChristianePalissa•BettinaEsch•SvenTwardziok•JulianeRieger•PaulWrede•MichaelF.G.Schmi

2、dtReceived:24July2012/Accepted:7October2012/Publishedonline:28November2012ÓSpringer-VerlagWien2012AbstractTheenteropathogeniccoronavirustransmissi-Introductionblegastroenteritisvirus(TGEV)causesseverediseaseinyoungpiglets.WehavestudiedtheprotectiveeffectsoftheTransmissiblegastroenteritisvir

3、us(TGEV)infectsentericprobioticEnterococcusfaeciumNCIMB10415(E.fae-andrespiratorytissuesandcausesseveregastroenteritiscium),whichisapprovedasafeedadditiveintheEuropeanwithamortalityratecloseto100%innewbornpiglets[3,Union,againstTGEVinfection.E.faeciumwasadded36].Theappearanceofthecloselyrel

4、atedTGEVvarianttoswinetesticle(ST)cellsbefore,concomitantlywith,porcinerespiratorycoronavirus(PRCoV)hasbeenfoundorafterTGEVinfection.ViabilityassaysrevealedthatbeneficialinpreventingTGEVinfections,possiblythroughE.faeciumledtoadose-dependentrescueofviabilityofinductionofneutralizingantibodie

5、sthatcanprovidecross-TGEV-infectedcellsreachingnearlytocompleteprotec-protectionagainstTGEVinfection[34,39].However,TGEtion.VirusyieldsoftheE.faeciumtreatedcultureswereprevalenceisstillbeingreported,andsomeTGEVstrainsreducedbyuptothreelog10units.Westernblotanalysisofhavebeenisolatedfromdome

6、sticpigsindifferentpartsofpurifiedTGEVrevealedthatthelevelsofallviralstructuraltheworld[28].Commerciallyavailablevaccines,eitherproteinswerereducedafterE.faeciumtreatment.Usinginactivatedorattenuated,havefailedtoprovidefullpro-transmissionelectronmicroscopy,weobservedattachmenttectiontopigle

7、ts[38].ItislikelythattheparentallyappliedofTGEVparticlestothesurfaceofE.faeciumwhichmightinactivatedvirusesdonotinducethelocalimmunebeameanstotrapvirusandtopreventinfection.Increasedresponseinthesmallintestinethatisrequiredforprotec-productionofnitricoxi