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1、©ELSEVIERAnn.Inst.Pasteur/Virol.Paris19871987,138,331-336EFFECTOFDEOXYCHOLATE,AMPHOTERICINBANDFONGIZONEONTRANSMISSmLEGASTROENTERITISCORONAVmUSbyT.D.Nguyen(*),E.BottreauandJ.M.Aynaud(**)INRA,LaboratoiredePathologiePorcine,37380Nouzilly(France)SUMMARYAta
2、concentrationof2ug/rnl,neitheramphotericinBnordeoxycholatehadaninactivatingeffectupontransmissiblegastroenteritiscoronavirusin•fectivity.However,amphothericinBstimulatedplaqueformatininagaroseandfacilitatedtheentryofviralRNAintoswinetestiscells.Thecomb
3、ina•tionofamphotericinB+deoxycholateinactivatedvirusinfectivityandinducedadecreaseinplaquediameter.Finally,inthepresenceoftheseagents,theproductionofinfectiousvirusandinterferonwasunchanged.KEY-WORDS:TOEvirus,Coronavirus,Fongizone,AmphotericinB,Deoxych
4、olate,Gastroenteritis;Swine,Invitro.INTRODUCTIONFongizoneisaclinicalformulationofamphotericinB(AmB)combinedwithdeoxycholate(DOC)asastabilizer,andhasbeenwidelyusedasanan•tifungalagent.TheantiviraleffectsofAmBresultnotonlyfromanaltera•tionoftheviralenvel
5、opebyitscombiningwithsterolcomponants[9]butalsofromenhancementofinterferon(IFN)productioninhostcells[2].HumancoronavirushasbeenfoundtobeinactivatedbyAmB[6].Ontheotherhand,AmBreportedlyenhancesviralRNAinfectivity[3].Sincecellculturesforvirologicalstudie
6、sveryoftenrequireantibioticsforpreventionoffunguscontamination,ouraimwastofurtherclarifytheeffectofthesecompounds,namelyfongizione,AmBandDOC,uponvirusinfectivityusingthetransmissi•blegastroenteritiscoronavirus(TOEV).SubmittedSeptember6,1986,accepted19M
7、arch,1987.(*)Presentaddress:NationalInstituteofVeterinaryResearch,NgaTuVong-DongDa,Hanoi(Vietnam).(**)Towhomallcorrespondenceshouldbeaddressed.332t:o.NGUYENANDCOLL.MATERIALSANDMETHODSChemicals.-Fongizone(Biopro,Mulhouse,France)wasusedasindicatedbythesu
8、pplierinthestateofitsdelivery.ArnB(Sigma,Mo,USA)wasdissolvedinethanolandusedimmediately.DOCwasfromSigma.DEAE-dextranwaspurcha•sedfromPharmacia(MW500,000)andproteinaseK(Boehringer,Mannheim,WesternGermany)wasdissolved(lmg/rnl)indistilledw
