冠状病毒Coronavirusl论文-1987 Primary structure of the glycoprotein E2 of coronavirus MHV-A59 and identification of the trypsin cleavage site.pdf

《冠状病毒Coronavirusl论文-1987 Primary structure of the glycoprotein E2 of coronavirus MHV-A59 and identification of the trypsin cleavage site.pdf》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。

1、VIROLOGY161,479-487(1987)PrimaryStructureoftheGlycoproteinE2ofCoronavirusMHV-A59andIdentificationoftheTrypsinCleavageSiteWILLEMLUYTJES,*LAWRENCES.STURMAN,tPETERJ.BREDENBEEK,”JEROENCHARITE,*BERNARDA.M.VANDERZEIJST,+MARIANC.HORZINEK,*ANDWlLLYJ.M.SPAAN*,’*InstituteofV

2、irology,VeterinaryFaculty,StateUniversityofUtrecht,3508TDUtrecht,TheNetherlands;tWadsworthCenterforLaboratoriesandResearch,NewYorkStateDepartmentofHealth,Albany,NewYork12201;and*SectionofBacteriology,VeterinaryFaculty,StateUniversityofUtrecht,TheNetherlandsReceived

3、March17,1987;acceptedAugust3,1987Thenucleotidesequenceofthepeplomer(EZ)geneofMHV-A59wasdeterminedfromasetofoverlappingcDNAclones.TheE2geneencodesaproteinof1324aminoacidsincludingahydrophobicsignalpeptide.AsecondlargehydrophobicdomainisfoundneartheCOOHterminusandpro

4、bablyrepresentsthemembraneanchor.Twentyglycosylationsitesarepredicted.CleavageoftheE2proteinresultsintwodifferent90Kspecies,90Aand906(L.S.Sturman,C.S.Ricard,andK.V.Holmes(1985)1.Viral56,904-91l),andactivatescellfusion.Proteinsequencingofthetrypsin-generatedN-termin

5、usrevealedthepositionofthecleavagesite.90Aand90BcouldbeidentifiedastheC-terminalandtheN-terminalparts,respectively.AminoacidsequencecomparisonoftheA59andJHME2proteinsshowedextensivehomoloav-_andrevealedastretchof89aminoacidsinthe9OBregionoftheA59E2proteinthatisabse

6、ntinJHM.o1987Academicpress.inc.INTRODUCTION90Kspecies(SturmanandHolmes,1977).Recentlyithasbeenshownthatthe90KproteinconsistsoftwoMurinehepatitisviruses(MHV)arecoronavirusesdifferentspecies,90Aand90B,arisingfromproteo-whichcauseavarietyofdiseasesincludinghepatitisly

7、ticcleavageofthe180Kprotein(Sturmanetal.,andencephalomyelitisinthenaturalhost(Wegeeta/.,1985).Thiscleavageactivatescellfusion,andtheratio1982).Theyarestudiedextensively,sinceMHVisaof180Kto90Kproteinsishostdependent(Sturmanetusefulanimalmodelforvirus-induceddemyelin

8、ationa/.,1985;Franaeta/.,1985).Ithasbeensuggestedandbecausecoronavirusespossessauniquemodeofthatsuchhost-dependentdifferencesintheprocess-replica