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1、VIRUSGENES2:3,293-294,1988GKluwerAcademicPublishers,ManufacturedinTheNetherlandsLettertotheEditorNucleotideSequenceBetweenthePeplomerandMatrixProteinGenesofthePorcineTransmissibleGastroenteritisCoronavirusIdentifiesThreeLargeOpenReadingFramesPAULA.KAPKE’,
2、FRANKY.T.TUNG*,ANDDAVIDA.BRIAN*‘UnitedStatesDepatimentofAgriculture,AgriculturalResearchSetvice,NationalAnimalDiseaseCenter,Ame.9,L4SOOlO,USA‘DepartmentofMicrobiology,TheUniversiyofTennessee,Knoxville,TN379960845,USAReceivedNovember9,1988AcceptedNovember1
3、0,1988RequestsforreprintsshouldbeaddressedtoPaulA.Kapke,U.S.DepartmentofAgriculture,Agricul-turalResearchService,NaturalAnimalDiseaseCenter.Ames,IA,50010,USAThenucleotidesequencebetweenthepeplomerandmatrixproteingenesinthegenomeofthePurduestrainofporcinet
4、ransmissiblegastroenteritiscoronavirus(TGEV)wasdeterminedbysequencingpartsofsixcDNAclones.Openreadingframespotentiallyencodingproteinsof7,711,27,711,and9,241Dawereidentified(Fig.1).ThesequenceforthisregionofthegenomeforthesamestrainofviruswaspublishedbyRa
5、sschaertetal.(3),butoursequencediffersbytwobases,oneofwhichresultsinamajorchangeinthepropertiesofthesecondopenreadingframe(ORF).Ginoursequenceatposition433,ratherthanT,enlargesthesecondopenreadingframefrom165to244aminoacidsandestablishesasequencecon-textm
6、orefavorableforinitiationoftranslation(4).Cinoursequenceatposition606,ratherthanT,changesLeutoPro.EachopenreadingframeisprecededbyasequencethatissimilartotheCTAAACintergenicsequencethoughttobere-quiredforleader-rimedtransciption(1,3).Theenlargementofthese
7、condopenreadingframefrom165aminoacids(18,833Da)to244aminoacids(27,711Da)resolvestwoconcernsraisedbyRasschaertetaL(3)namelya)thesecondORFintheirsequencewouldrequireinitiationoftranslation570basesdownstreamfromtheCTAAACintergenicsequence(or249basesdownstrea
8、moftheCTAAATse-quencethatweproposeisused),whichisanunusuallylongdistance.b)Thesec-ondORFintheirsequenceisnotlargeenoughtoencodethe24kDpolypeptidetranslatedinvitrofromTGEVmRNA3byJacobsetal.(5)anmRNA294KAPKE,TUNG.ANDB
