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1、VirusResearch,13(1989)87-10087ElsevierVRR00499NucleotidesequenceofcoronavirusTGEVgenomicRNA:evidencefor3mRNAspeciesbetweenthepeplomerandmatrixproteingenesRonaldD.Wesley,AndrewK.Cheung,DavidD.MichaelandRogerD.WoodsUS?..&AgriculturalResearchService,NationalAnimalDiseaseCenter,P.0.Box70,Ames,
2、Iowa,U.S.A.(Accepted30January1989)TheregionoftheTGEVgenomebetweentheEl-matrixproteingeneandtheE2-peplomerproteingenehasbeensequencedfromacDNAclone.Theconsensusrecognitionsequence,5’gCTAAACwasfoundupstreamfrom3largeopenreadingframes.Incoronavirusesthesehomologousrecognitionsequencesareinvol
3、vedintheiuitiationoftranscriptionsuggestingthatthereare3mRNAspeciesinthisregionoftheTGEVgenome.NorthernblotanalysisandnucleaseSlmappingconfirmedthepresenceof3mRNAspeciesbetweenmRNA3encodingtheEZ-peplomerproteinandmRNA6encodingtheEl-matrixprotein.The5’regionsofthese3mRNAsencodepotentialpoly
4、peptidesofpredictedmolecularweight;7859,27744and9287,respectively.ThepotentialtranslationproductofORFB(27744Da)isconsiderablylargerthanpreviouslyreportedandcouldbedifficulttodistinguishbysizefromtheEl-matrixprotein.Coronavirus;TGEV;RNAsequencingTransmissiblegastroenteritisvirus(TGEV)isanec
5、onomicallyimportantcoronavirusofswinethatproducesanoftenfataldiarrheaespeciallyinnursingpigsCorrespondencero:R.D.Wesley,NationalAnimalDiseaseCenter,P.O.Box70,Ames,IA50010,U.S.A.016%1702/89/$03.5001989ElsevierSciencePublishersB.V.(BiomedicalDivision)88lessthan2weeksofage(SaifandBohl,1986).L
6、ikeothermembersofthefamilyCoronaviridae,TGEvirionsconsistof3majorstructuralproteins;theEl-matrixandEZpeplomersurfaceglycoproteinsandaphosphorylatednucleocapsidprotein(N)thatassociateswiththe23.6kilobase(kb),non-segmented,positive-strandedRNAgenome(GarwesandPocock,1975;Brianetal.,1984;Hueta
7、l.,1984;Jacobsetal.,1986;WesleyandWoods,1986).ThenucleotideanddeducedaminoacidsequencesofthesestructuralproteinshavebeendeterminedfortheavirulentPurdue115strainofTGEV(KapkeandBrian,1986;Laudeetal.,1987;RasschaertandLaude,1987).InthereplicationofTGEV,aswithothe
