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《冠状病毒Coronavirusl论文-1986 Coronavirus MHV-JHM_ nucleotide sequence of the mRNA that encodes the membrane protein.pdf》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。
1、volume14Number151986NucleicAcidsResearchCoronavirusMHV-JHM:nucleotidesequenceofthemRNAthatencodesthemembraneprotein1M.Pfleiderer,M.A.SkinnerandS.G.SiddellInstituteofVirology,VersbacherStr.7,8700Wiirzburg,FRGand'DepartmentofMicrobiology,UniversityofReading,ReadingRG15AQ,UKSubmitted26June1986Thecompl
2、etesequenceofthemembraneproteinofcoronavirusMHV-JHMispresentedandcomparedwiththatofMHV-A59(1).Withinthecodingregionof684nucleotidesthereare21nucleotidechangeswhichresultin7aminoacidchangesinaproteinof228residues(tnol.wt.26,000).Theseaminoacidchangesareconservative,bothwithinthepotentialmembrane()an
3、dnon-membranedomainsoftheprotein.ThepotentialO-glycosy-lationsites(V)attheN-terminusofthepolypeptidearealsoconserved.Inthe5'non-codingregion,thehomologysequenceTCTAATCCAAACisfoundatornearthesiteoffusionbetweenthemRNAbodyandleader.1TGGCGTCCGTACGTACCCTCTCTACTCTAAAACTCTTGTAGTTTAAATCTAATCTAATCC60.VVVTV
4、.A.T.G61AAACATTATGAGTAGTACCACTCAGGCCCCAGGGCCCGTCTACCAATGGACAGCCGACGA120MSSTTQAPGPVYQVTADE121GGCCGTTCAATTCCTTAAGGAATGGAACTTCTCGTTGGGCATTATACTACTCTTTATTAC180AVQFLKEWNFSLGIILLPIT181TATCATACTACAGTTCGGTTACACGAGCCGTAGCATGTTTATCTATGTTGTGAAMTGAT240IILQFGYTSRSHFIYVVKMIC..T.T241AATCTTGTGGTTAATGTGGCCACTGATTAT
5、TGTTTTGTGTATGTTCAATTGCGTGTATGC300ILWLHWPLIIVLCMFNCVYA301GCTAAATAAfGTGTATCTTGGATTTTCTATAGTGTTTACTATAGTGTCCGTTGTAATGTG360LNNVYLGFSIVFTIVSVVMW361GATTATGTATTTrGTTMTAGCATCAGGTTGTTTATCAGGACTGGTAGCTGGTGGAGCTT420IMYFVNSIRLFIRTGSWWSF'..TT.421CAACCCCGAAACAAACAACCTAATGTGCATAGATATGAAAGGTACCGTGTATGTTAGACC480NPE
6、TNNLHCIDMKGTVYVRPCC481CATTATTGAGGATTACCATACAaAACAGCCACTATTATTCGTGGCCACTTCTATATGCA540IIEDYHTLTATIIRGHFYMQ.C.T.541AGGTGTTMGCTAGGCACCCKSTTrCTCTTTGTCTGACTTGCCTGCTTATGTTACAGTAGC600GVKLGTGFSLSDLPAYVTVAGA..C.601TAAGGTTTCCCACCTTTGCACTTATAAGCGTGCATTCTTAGACAAGGTAGACGGTGTTAG660KVSHLCTYKRAFLDKVDGVS661CGGTTrrGC
7、TGTTTATGTGAAGTCCAAGGfcGGAAATTACCGACTGCCCtCAAACAAACC720GFAVYVKSKVGNYRLPSNKPC.721GAGTGGCGCGGACACCGTATTGTTGAGMTCTMTCrAMCTTrMGGATGTCTTTTGTT780SGADTVLLRI1.Armstrong,J.,Niemann,H.,Smeekens,S.,Rottier,P.andWarren,
